BMRB Entry 51448
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51448
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Title: HigA2
Deposition date: 2022-05-19 Original release date: 2022-06-20
Authors: Kovacic, Matic
Citation: Hadzi, San; Kovacic, Matic; Plavec, Janez; Volkov, Alexander; Loris, Remy. "Fuzzy DNA recognition by a prokaryotic transcription factor" .
Assembly members:
entity_1, polymer, 104 residues, 11700 Da.
Natural source: Common Name: Vibrio Cholerae Taxonomy ID: 666 Superkingdom: Bacteria Kingdom: not available Genus/species: Vibrio cholerae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet15
Entity Sequences (FASTA):
entity_1: MSNRDLFAELSSALVEAKQH
SEGKLTLKTHHVNDVGELNI
SPDEIVSIREQFNMSRGVFA
RLLHTSSRTLENWEQGRSVP
NGQAVTLLKLVQRHPETLSH
IAEL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 280 |
| 15N chemical shifts | 94 |
| 1H chemical shifts | 94 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HigA2, chain 1 | 1 |
| 2 | HigA2, chain 2 | 1 |
Entities:
Entity 1, HigA2, chain 1 104 residues - 11700 Da.
| 1 | MET | SER | ASN | ARG | ASP | LEU | PHE | ALA | GLU | LEU | ||||
| 2 | SER | SER | ALA | LEU | VAL | GLU | ALA | LYS | GLN | HIS | ||||
| 3 | SER | GLU | GLY | LYS | LEU | THR | LEU | LYS | THR | HIS | ||||
| 4 | HIS | VAL | ASN | ASP | VAL | GLY | GLU | LEU | ASN | ILE | ||||
| 5 | SER | PRO | ASP | GLU | ILE | VAL | SER | ILE | ARG | GLU | ||||
| 6 | GLN | PHE | ASN | MET | SER | ARG | GLY | VAL | PHE | ALA | ||||
| 7 | ARG | LEU | LEU | HIS | THR | SER | SER | ARG | THR | LEU | ||||
| 8 | GLU | ASN | TRP | GLU | GLN | GLY | ARG | SER | VAL | PRO | ||||
| 9 | ASN | GLY | GLN | ALA | VAL | THR | LEU | LEU | LYS | LEU | ||||
| 10 | VAL | GLN | ARG | HIS | PRO | GLU | THR | LEU | SER | HIS | ||||
| 11 | ILE | ALA | GLU | LEU |
Samples:
sample_1: HigA2 homodimer antitoxin protein, [U-100% 13C; U-100% 15N], 250 uM; sodium acetate 10 mM; arginine 10 mM; glutamate 10 mM
sample_conditions_1: ionic strength: 50 mM; pH: 4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA v1.9.1.7 - chemical shift assignment
NMRFAM-SPARKY - data analysis
VNMRj v3.2A - collection
NMRPipe - processing
NMR spectrometers:
- Varian VNMRS 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts