BMRB Entry 51473
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51473
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: IL1R8 PubMed: 35889366
Deposition date: 2022-05-24 Original release date: 2022-08-08
Authors: Lee, Eunjeong
Citation: Lee, Eunjeong; Redzic, Jasmina; Nemkov, Travis; Saviola, Anthony; Dzieciatkowska, Monika; Hansen, Kirk; D'Alessandro, Angelo; Dinarello, Charles; Eisenmesser, Elan. "Human and Bacterial Toll-Interleukin Receptor Domains Exhibit Distinct Dynamic Features and Functions" Molecules 27, 4494-4494 (2022).
Assembly members:
entity_1, polymer, 150 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet27
Entity Sequences (FASTA):
entity_1: DGKLYDAYVSYSDCPEDRKF
VNFILKPQLERRRGYKLFLD
DRDLLPRAEPSADLLVNLSR
CRRLIVVLSDAFLSRAWCSH
SFREGLCRLLELTRRPIFIT
FEGQRRDPAHPALRLLRQHR
HLVTLLLWRPGSVTPSSDFW
KEVQLALPRK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 369 |
15N chemical shifts | 125 |
1H chemical shifts | 125 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | IL1R8 | 1 |
Entities:
Entity 1, IL1R8 150 residues - Formula weight is not available
1 | ASP | GLY | LYS | LEU | TYR | ASP | ALA | TYR | VAL | SER | |
2 | TYR | SER | ASP | CYS | PRO | GLU | ASP | ARG | LYS | PHE | |
3 | VAL | ASN | PHE | ILE | LEU | LYS | PRO | GLN | LEU | GLU | |
4 | ARG | ARG | ARG | GLY | TYR | LYS | LEU | PHE | LEU | ASP | |
5 | ASP | ARG | ASP | LEU | LEU | PRO | ARG | ALA | GLU | PRO | |
6 | SER | ALA | ASP | LEU | LEU | VAL | ASN | LEU | SER | ARG | |
7 | CYS | ARG | ARG | LEU | ILE | VAL | VAL | LEU | SER | ASP | |
8 | ALA | PHE | LEU | SER | ARG | ALA | TRP | CYS | SER | HIS | |
9 | SER | PHE | ARG | GLU | GLY | LEU | CYS | ARG | LEU | LEU | |
10 | GLU | LEU | THR | ARG | ARG | PRO | ILE | PHE | ILE | THR | |
11 | PHE | GLU | GLY | GLN | ARG | ARG | ASP | PRO | ALA | HIS | |
12 | PRO | ALA | LEU | ARG | LEU | LEU | ARG | GLN | HIS | ARG | |
13 | HIS | LEU | VAL | THR | LEU | LEU | LEU | TRP | ARG | PRO | |
14 | GLY | SER | VAL | THR | PRO | SER | SER | ASP | PHE | TRP | |
15 | LYS | GLU | VAL | GLN | LEU | ALA | LEU | PRO | ARG | LYS |
Samples:
sample_1: IL1R8, [U-13C; U-15N], 1 mM; DTT 1 mM; HEPES 50 mM; sodium chloride 150 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts