BMRB Entry 51475
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51475
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 53BP1 1140-1225 PubMed: 36335430
Deposition date: 2022-05-26 Original release date: 2022-12-12
Authors: Howe, Jesse; Weeks, Austin; Reardon, Patrick; Barbar, Elisar
Citation: Howe, Jesse; Weeks, Austin; Reardon, Patrick; Barbar, Elisar. "Multivalent binding of the hub protein LC8 at a newly discovered site in 53BP1" Biophys. J. 121, 4433-4442 (2022).
Assembly members:
entity_1, polymer, 89 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24d
Entity Sequences (FASTA):
entity_1: GAHSKALIERPSQNNIGIQT
MESSLRVPETVSAATQTIKN
VSEQGTSTVDQNFGKQDATV
QTERGSGEKPVSAPGDDTES
LHSQGEEEF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 207 |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 73 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 53BP1 | 1 |
Entities:
Entity 1, 53BP1 89 residues - Formula weight is not available
| 1 | GLY | ALA | HIS | SER | LYS | ALA | LEU | ILE | GLU | ARG | ||||
| 2 | PRO | SER | GLN | ASN | ASN | ILE | GLY | ILE | GLN | THR | ||||
| 3 | MET | GLU | SER | SER | LEU | ARG | VAL | PRO | GLU | THR | ||||
| 4 | VAL | SER | ALA | ALA | THR | GLN | THR | ILE | LYS | ASN | ||||
| 5 | VAL | SER | GLU | GLN | GLY | THR | SER | THR | VAL | ASP | ||||
| 6 | GLN | ASN | PHE | GLY | LYS | GLN | ASP | ALA | THR | VAL | ||||
| 7 | GLN | THR | GLU | ARG | GLY | SER | GLY | GLU | LYS | PRO | ||||
| 8 | VAL | SER | ALA | PRO | GLY | ASP | ASP | THR | GLU | SER | ||||
| 9 | LEU | HIS | SER | GLN | GLY | GLU | GLU | GLU | PHE |
Samples:
sample_1: 53BP1, [U-100% 15N], 200 uM
sample_conditions_1: ionic strength: 0.070 M; pH: 6.5; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRViewJ - chemical shift assignment
NMRPipe - processing
TOPSPIN - collection
NMR spectrometers:
- Bruker AVANCE III HD 800 MHz MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts