BMRB Entry 51481
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51481
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Title: Chemical Shift Assignments of CT-IC 216-260 PubMed: 36416224
Deposition date: 2022-06-03 Original release date: 2022-12-12
Authors: Loening, Nikolaus
Citation: Jara, Kayla; Loening, Nikolaus; Reardon, Patrick; Yu, Zhen; Woonnimani, Prajna; Brooks, Coban; Vesely, Cat; Barbar, Elisar. "Multivalency, autoinhibition, and protein disorder in the regulation of interactions of dynein intermediate chain with dynactin and the nuclear distribution protein" Elife 11, e80217-e80217 (2022).
Assembly members:
entity_1, polymer, 46 residues, Formula weight is not available
Natural source: Common Name: ascomycete fungi Taxonomy ID: 209285 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Thermochaetoides thermophila
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-24
Entity Sequences (FASTA):
entity_1: GAEELDAVTKSVEFMDFIDR
STKVIEKALDQEYDILTDYT
LQVHDV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 156 |
| 15N chemical shifts | 46 |
| 1H chemical shifts | 322 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CTIC 216-260 | 1 |
Entities:
Entity 1, CTIC 216-260 46 residues - Formula weight is not available
First residue (G) is a non-native artifact
| 1 | GLY | ALA | GLU | GLU | LEU | ASP | ALA | VAL | THR | LYS | ||||
| 2 | SER | VAL | GLU | PHE | MET | ASP | PHE | ILE | ASP | ARG | ||||
| 3 | SER | THR | LYS | VAL | ILE | GLU | LYS | ALA | LEU | ASP | ||||
| 4 | GLN | GLU | TYR | ASP | ILE | LEU | THR | ASP | TYR | THR | ||||
| 5 | LEU | GLN | VAL | HIS | ASP | VAL |
Samples:
sample_1: CT-IC 216-260, [U-99% 15N], 550 uM; DSS 200 uM; sodium chloride 20 mM; sodium phosphate 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 283.1 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.1.4 - collection, processing
CcpNMR v2.5.2 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts