BMRB Entry 51485
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51485
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Title: Chemical shift assignments of the C-terminal domain of AKAP5 (AKAP79), residues 300-427 PubMed: 35697294
Deposition date: 2022-06-09 Original release date: 2022-06-14
Authors: Watson, Matthew; Almeida, Teresa; Ray, Arundhati; Hanack, Christina; Elston, Rory; Btesh, Joan; McNaughton, Peter; Stott, Katherine
Citation: Watson, Matthew; Almeida, Teresa; Ray, Arundhati; Hanack, Christina; Elston, Rory; Btesh, Joan; McNaughton, Peter; Stott, Katherine. "Hidden Multivalency in Phosphatase Recruitment by a Disordered AKAP Scaffold" J. Mol. Biol. 434, 167682-167682 (2022).
Assembly members:
entity_1, polymer, 130 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHAT3 AKAP5c
Entity Sequences (FASTA):
entity_1: GSVAEETKPKDTELSQESDF
KENGITEEKSKSEESKRMEP
IAIIITDTEISEFDVTKSKN
VPKQFLISAENEQVGVFAND
NGFEDRTSEQYETLLIETAS
SLVKNAIQLSIEQLVNEMAS
DDNKINNLLQ
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 125 |
| 1H chemical shifts | 125 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AKAP5 (AKAP79) | 1 |
Entities:
Entity 1, AKAP5 (AKAP79) 130 residues - Formula weight is not available
| 1 | GLY | SER | VAL | ALA | GLU | GLU | THR | LYS | PRO | LYS | |
| 2 | ASP | THR | GLU | LEU | SER | GLN | GLU | SER | ASP | PHE | |
| 3 | LYS | GLU | ASN | GLY | ILE | THR | GLU | GLU | LYS | SER | |
| 4 | LYS | SER | GLU | GLU | SER | LYS | ARG | MET | GLU | PRO | |
| 5 | ILE | ALA | ILE | ILE | ILE | THR | ASP | THR | GLU | ILE | |
| 6 | SER | GLU | PHE | ASP | VAL | THR | LYS | SER | LYS | ASN | |
| 7 | VAL | PRO | LYS | GLN | PHE | LEU | ILE | SER | ALA | GLU | |
| 8 | ASN | GLU | GLN | VAL | GLY | VAL | PHE | ALA | ASN | ASP | |
| 9 | ASN | GLY | PHE | GLU | ASP | ARG | THR | SER | GLU | GLN | |
| 10 | TYR | GLU | THR | LEU | LEU | ILE | GLU | THR | ALA | SER | |
| 11 | SER | LEU | VAL | LYS | ASN | ALA | ILE | GLN | LEU | SER | |
| 12 | ILE | GLU | GLN | LEU | VAL | ASN | GLU | MET | ALA | SER | |
| 13 | ASP | ASP | ASN | LYS | ILE | ASN | ASN | LEU | LEU | GLN |
Samples:
sample_1: AKAP5 (AKAP79), [U-98% 15N], 50-100 uM; PIPES 10 mM; NaCl 150 mM
sample_conditions_1: ionic strength: 0.16 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
AZARA v2.7 - processing
ANALYSIS v2.4 - chemical shift assignment
NMR spectrometers:
- Bruker DRX 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts