BMRB Entry 51488

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51488

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone assignment of the LCC PETase
Deposition date:
2022-06-13
Original release date:
2022-08-08
Authors:
Charlier, Cyril; Lippens, Guy
Citation:

Citation: Charlier, Cyril; Gavalda, Sabine; Borsenberger, Vinciane; Duquesne, Sophie; Marty, Alain; Tournier, Vincent; Lippens, Guy. "An NMR look at an engineered PET depolymerase"  Biophys. J. 121, 2882-2894 (2022).
PubMed: 35794828

Assembly members:

Assembly members:
entity_1, polymer, 258 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: uncultured bacterium   Taxonomy ID: 77133   Superkingdom: Bacteria   Kingdom: not available   Genus/species: uncultured bacterium

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-26b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SNPYQRGPNPTRSALTADGP FSVATYTVSRLSVSGFGGGV IYYPTGTSLTFGGIAMSPGY TADASSLAWLGRRLASHGFV VLVINTNSRFDYPDSRASQL SAALNYLRTSSPSAVRARLD ANRLAVAGHAMGGGGTLRIA EQNPSLKAAVPLTPWHTDKT FNTSVPVLIVGAEADTVAPV SQHAIPFYQNLPSTTPKVYV ELDNASHFAPNSNNAAISVY TISWMKLWVDNDTRYRQFLC NVNDPALSDFRTNNRHCQ

Data sets:
Data typeCount
13C chemical shifts647
15N chemical shifts204
1H chemical shifts204

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LCC PETase1

Entities:

Entity 1, LCC PETase 258 residues - Formula weight is not available

1   SERASNPROTYRGLNARGGLYPROASNPRO
2   THRARGSERALALEUTHRALAASPGLYPRO
3   PHESERVALALATHRTYRTHRVALSERARG
4   LEUSERVALSERGLYPHEGLYGLYGLYVAL
5   ILETYRTYRPROTHRGLYTHRSERLEUTHR
6   PHEGLYGLYILEALAMETSERPROGLYTYR
7   THRALAASPALASERSERLEUALATRPLEU
8   GLYARGARGLEUALASERHISGLYPHEVAL
9   VALLEUVALILEASNTHRASNSERARGPHE
10   ASPTYRPROASPSERARGALASERGLNLEU
11   SERALAALALEUASNTYRLEUARGTHRSER
12   SERPROSERALAVALARGALAARGLEUASP
13   ALAASNARGLEUALAVALALAGLYHISALA
14   METGLYGLYGLYGLYTHRLEUARGILEALA
15   GLUGLNASNPROSERLEULYSALAALAVAL
16   PROLEUTHRPROTRPHISTHRASPLYSTHR
17   PHEASNTHRSERVALPROVALLEUILEVAL
18   GLYALAGLUALAASPTHRVALALAPROVAL
19   SERGLNHISALAILEPROPHETYRGLNASN
20   LEUPROSERTHRTHRPROLYSVALTYRVAL
21   GLULEUASPASNALASERHISPHEALAPRO
22   ASNSERASNASNALAALAILESERVALTYR
23   THRILESERTRPMETLYSLEUTRPVALASP
24   ASNASPTHRARGTYRARGGLNPHELEUCYS
25   ASNVALASNASPPROALALEUSERASPPHE
26   ARGTHRASNASNARGHISCYSGLN

Related Database Links:

PDB 6THS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks