BMRB Entry 51489

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51489

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Title:
NMR assignment of the mCherry protein
Deposition date:
2022-06-14
Original release date:
2023-07-28
Authors:
Sette, Marco; Johnson, Laura Anne; Friis, Pia; Pardi, Arthur; Jimenez, Ralph; Mulder, Frans
Citation:

Citation: Sette, Marco; Johnson, Laura Anne; Jimenez, Ralph; Mulder, Frans. "Backbone 1H, 15N and 13C resonance assignments of the 27kDa fluorescent protein mCherry"  Biomol. NMR Assign. 17, 243-247 (2023).
PubMed: 37684490

Assembly members:

Assembly members:
entity_1, polymer, 242 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: coral anemones   Taxonomy ID: 86600   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Discosoma Discosoma sp.

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGHHHHHHGVSKGEEDNMAI IKEFMRFKVHMEGSVNGHEF EIEGEGEGRPYEGTQTAKLK VTKGGPLPFAWDILSPQFMY GSKAYVKHPADIPDYLKLSF PEGFKWERVMNFEDGGVVTV TQDSSLQDGEFIYKVKLRGT NFPSDGPVMQKKTMGWEASS ERMYPEDGALKGEIKQRLKL KDGGHYDAEVKTTYKAKKPV QLPGAYNVNIKLDITSHNED YTIVEQYERAEGRHSTGGMD ELYK

Data sets:
Data typeCount
13C chemical shifts608
15N chemical shifts199
1H chemical shifts199

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1mCherry1

Entities:

Entity 1, mCherry 242 residues - Formula weight is not available

1   METGLYHISHISHISHISHISHISGLYVAL
2   SERLYSGLYGLUGLUASPASNMETALAILE
3   ILELYSGLUPHEMETARGPHELYSVALHIS
4   METGLUGLYSERVALASNGLYHISGLUPHE
5   GLUILEGLUGLYGLUGLYGLUGLYARGPRO
6   TYRGLUGLYTHRGLNTHRALALYSLEULYS
7   VALTHRLYSGLYGLYPROLEUPROPHEALA
8   TRPASPILELEUSERPROGLNPHEMETTYR
9   GLYSERLYSALATYRVALLYSHISPROALA
10   ASPILEPROASPTYRLEULYSLEUSERPHE
11   PROGLUGLYPHELYSTRPGLUARGVALMET
12   ASNPHEGLUASPGLYGLYVALVALTHRVAL
13   THRGLNASPSERSERLEUGLNASPGLYGLU
14   PHEILETYRLYSVALLYSLEUARGGLYTHR
15   ASNPHEPROSERASPGLYPROVALMETGLN
16   LYSLYSTHRMETGLYTRPGLUALASERSER
17   GLUARGMETTYRPROGLUASPGLYALALEU
18   LYSGLYGLUILELYSGLNARGLEULYSLEU
19   LYSASPGLYGLYHISTYRASPALAGLUVAL
20   LYSTHRTHRTYRLYSALALYSLYSPROVAL
21   GLNLEUPROGLYALATYRASNVALASNILE
22   LYSLEUASPILETHRSERHISASNGLUASP
23   TYRTHRILEVALGLUGLNTYRGLUARGALA
24   GLUGLYARGHISSERTHRGLYGLYMETASP
25   GLULEUTYRLYS

Related Database Links:

PDB 2h5q

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks