BMRB Entry 51504

Name: Assignment of 1-44 residues of nucleocapsid protein of SARS-CoV-2
Published: 2022-09-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51504

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Assignment of 1-44 residues of nucleocapsid protein of SARS-CoV-2 PubMed: 36040256

Deposition date: 2022-06-27 Original release date: 2022-09-13

Authors: Ibanez de Opakua, Alain; Zweckstetter, Markus; Zachrdla, Milan

Citation: Zachrdla, Milan; Savastano, Adriana; Ibanez de Opakua, Alain; Cima-Omori, Sol; Zweckstetter, Markus. "Contributions of the N-terminal intrinsically disordered region of the severe acute respiratory syndrome coronavirus 2 nucleocapsid protein to RNA-induced phase separation" Protein Sci. 31, e4409-e4409 (2022).

Assembly members:
entity_1, polymer, 44 residues, Formula weight is not available

Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: MSDNGPQNQRNAPRITFGGP SDSTGSNQNGERSGARSKQR RPQG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	81
15N chemical shifts	38
1H chemical shifts	90

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	peptide	1

Entities:

Entity 1, peptide 44 residues - Formula weight is not available

1

MET

SER

ASP

ASN

GLY

PRO

GLN

ASN

GLN

ARG

2

ASN

ALA

PRO

ARG

ILE

THR

PHE

GLY

PRO

3

SER

ASP

SER

THR

GLY

SER

ASN

GLN

ASN

GLY

4

GLU

ARG

SER

GLY

ALA

ARG

SER

LYS

GLN

ARG

5

ARG

PRO

GLN

GLY

Samples:

sample_1: 1-44 residues of nucleocapsid protein of SARS-CoV-2 2 mM; D2O, [U-2H], 5%; sodium azide 0.01%; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection, processing

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts