BMRB Entry 51505

Name: Backbone 1H, 13C and 15N Assignments of MAX(1-21)
Published: 2022-10-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51505

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Backbone 1H, 13C and 15N Assignments of MAX(1-21) PubMed: 36174765

Deposition date: 2022-06-28 Original release date: 2022-10-24

Authors: Schutz, Stefan; Jahnke, Wolfgang

Citation: Schutz, Stefan; Bergsdorf, Christian; Goretzki, Benedikt; Lingel, Andreas; Renatus, Martin; Gossert, Alvar; Jahnke, Wolfgang. "The disordered MAX N-terminus modulates DNA binding of the transcription factor MYC:MAX" J. Mol. Biol. 434, 167833-167833 (2022).

Assembly members:
entity_1, polymer, 21 residues, 2434.4 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM41-like

Entity Sequences (FASTA):
entity_1: MSDNDDIEVESDEEQPRFQS A

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	87
15N chemical shifts	24
1H chemical shifts	24

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MAX(1-21), trans	1
2	MAX(1-21), cis	1

Entities:

Entity 1, MAX(1-21), trans 21 residues - 2434.4 Da.

1

MET

SER

ASP

ASN

ASP

ILE

GLU

VAL

GLU

2

SER

ASP

GLU

GLN

PRO

ARG

PHE

GLN

SER

3

ALA

Samples:

sample_1: MAX(1-21), [U-99% 13C; U-99% 15N], 1.05 mM; D2O, [U-99% 2H], 5 v/v; HEPES 50 mM; sodium chloride 100 mM; TCEP 2 mM; magnesium chloride 20 mM; EDTA 20 mM; ATP 5 mM

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.2 - collection, data analysis, processing

CcpNMR v3.0 - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts