BMRB Entry 51512
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51512
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Title: Backbone 1H, 13C, and 15N chemical shift assignments for the chromoshadow domain (residue 112-176) of human heterochromatin protein 1a (HP1a). PubMed: 36537242
Deposition date: 2022-07-08 Original release date: 2023-06-30
Authors: Her, Cheenou; Debelouchina, Galia
Citation: Her, Cheenou; Phan, Tien; Jovic, Nina; Kapoor, Utkarsh; Ackermann, Bryce; Rizuan, Azamat; Kim, Young; Mittal, Jeetain; Debelouchina, Galia. "Molecular interactions underlying the phase separation of HP1a: role of phosphorylation, ligand and nucleic acid binding" Nucleic Acids Res. 50, 12702-12722 (2022).
Assembly members:
entity_1, polymer, 66 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET (2B-T)
Entity Sequences (FASTA):
entity_1: SDIARGFERGLEPEKIIGAT
DSCGDLMFLMKWKDTDEADL
VLAKEANVKCPQIVIAFYEE
RLTWHA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 126 |
| 15N chemical shifts | 62 |
| 1H chemical shifts | 62 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HP1a-CSD | 1 |
Entities:
Entity 1, HP1a-CSD 66 residues - Formula weight is not available
| 1 | SER | ASP | ILE | ALA | ARG | GLY | PHE | GLU | ARG | GLY | ||||
| 2 | LEU | GLU | PRO | GLU | LYS | ILE | ILE | GLY | ALA | THR | ||||
| 3 | ASP | SER | CYS | GLY | ASP | LEU | MET | PHE | LEU | MET | ||||
| 4 | LYS | TRP | LYS | ASP | THR | ASP | GLU | ALA | ASP | LEU | ||||
| 5 | VAL | LEU | ALA | LYS | GLU | ALA | ASN | VAL | LYS | CYS | ||||
| 6 | PRO | GLN | ILE | VAL | ILE | ALA | PHE | TYR | GLU | GLU | ||||
| 7 | ARG | LEU | THR | TRP | HIS | ALA |
Samples:
sample_1: potassium chloride 75 mM; TCEP 1 mM; HEPES 20 mM; sodium azide 0.05%; Chromoshadow domain of human heterochromatin protein 1a (HP1a), [U-99% 13C; U-99% 15N], 400 uM
sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts