BMRB Entry 51516
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51516
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Title: 1H, 13C, 15N NMR assignment of Engrailed2 homeodomain in anionic bicelles PubMed: 35988722
Deposition date: 2022-07-16 Original release date: 2022-10-31
Authors: Carlier, Ludovic; LEQUIN, Olivier
Citation: Carlier, Ludovic; Samson, Damien; Khemtemourian, Lucie; Joliot, Alain; Fuchs, Patrick; Lequin, Olivier. "Anionic lipids induce a fold-unfold transition in the membrane-translocating Engrailed homeodomain" Biochim. Biophys. Acta Biomembr. 1864, 184030-184030 (2022).
Assembly members:
entity_1, polymer, 64 residues, Formula weight is not available
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSCherry
Entity Sequences (FASTA):
entity_1: GPASDKRPRTAFTAEQLQRL
KAEFQTNRYLTEQRRQSLAQ
ELGLNESQIKIWFQNKRAKI
KKAT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 176 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 241 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Engrailed 2 homeodomain | 1 |
Entities:
Entity 1, Engrailed 2 homeodomain 64 residues - Formula weight is not available
Residues 200-259 of chicken Engrailed 2 homeodomain (UniProt Q05917) plus N-terminal extension GPAS.
| 1 | GLY | PRO | ALA | SER | ASP | LYS | ARG | PRO | ARG | THR | ||||
| 2 | ALA | PHE | THR | ALA | GLU | GLN | LEU | GLN | ARG | LEU | ||||
| 3 | LYS | ALA | GLU | PHE | GLN | THR | ASN | ARG | TYR | LEU | ||||
| 4 | THR | GLU | GLN | ARG | ARG | GLN | SER | LEU | ALA | GLN | ||||
| 5 | GLU | LEU | GLY | LEU | ASN | GLU | SER | GLN | ILE | LYS | ||||
| 6 | ILE | TRP | PHE | GLN | ASN | LYS | ARG | ALA | LYS | ILE | ||||
| 7 | LYS | LYS | ALA | THR |
Samples:
sample_1: Engrailed 2 homeodomain, [U-100% 13C; U-100% 15N], 1 mM; DHPC 50 mM; DMPG 25 mM; sodium phosphate 30 mM; sodium chloride 70 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 500 MHz
Related Database Links:
| UniprotDB | Q05917 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts