BMRB Entry 51518

Name: CaBP1 C-Lobe Bound to CaV1.2 IQ-motif
Published: 2022-09-13

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51518

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: CaBP1 C-Lobe Bound to CaV1.2 IQ-motif PubMed: 36064846

Deposition date: 2022-07-19 Original release date: 2022-09-13

Authors: Salveson, Ian; Ames, James

Citation: Salveson, Ian; Ames, James. "Chemical shift assignments of the C-terminal domain of CaBP1 bound to the IQ-motif of voltage-gated Ca 2+ channel (Ca V 1.2)" Biomol. NMR Assignments 16, 385-390 (2022).

Assembly members:
entity_1, polymer, 70 residues, Formula weight is not available
entity_2, polymer, 18 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET 3a

Entity Sequences (FASTA):
entity_1: MIGVKELRDAFREFDTNGDG EISTSELREAMRKLLGHQVG HRDIEEIIRDVDLNGDGRVD FEEFVRMMSR
entity_2: KFYATFLIQEYFRKFKKR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	169
15N chemical shifts	54
1H chemical shifts	220

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CaBP1 C-lobe	1
2	IQ-motif	2
3	Calcium, 1	3
4	Calcium, 2	3

Entities:

Entity 1, CaBP1 C-lobe 70 residues - Formula weight is not available

1	MET	ILE	GLY	VAL	LYS	GLU	LEU	ARG	ASP	ALA
2	PHE	ARG	GLU	PHE	ASP	THR	ASN	GLY	ASP	GLY
3	GLU	ILE	SER	THR	SER	GLU	LEU	ARG	GLU	ALA
4	MET	ARG	LYS	LEU	LEU	GLY	HIS	GLN	VAL	GLY
5	HIS	ARG	ASP	ILE	GLU	GLU	ILE	ILE	ARG	ASP
6	VAL	ASP	LEU	ASN	GLY	ASP	GLY	ARG	VAL	ASP
7	PHE	GLU	GLU	PHE	VAL	ARG	MET	MET	SER	ARG

Entity 2, IQ-motif 18 residues - Formula weight is not available

1

LYS

PHE

TYR

ALA

THR

PHE

LEU

ILE

GLN

GLU

2

TYR

PHE

ARG

LYS

PHE

LYS

ARG

Entity 3, Calcium, 1 - Ca - 40.078 Da.

1

CA

Samples:

sample_1: CaBP1 C-Lobe, [U-100% 15N], 0.5 mM; CaV1.2 IQ-motif 0.75 mM; Ca2+ 1 mM; Tris 10 mM

sample_2: CaBP1 C-Lobe, [U-100% 13C; U-100% 15N], 0.6 mM; CaV1.2 IQ-motif 0.9 mM; Ca2+ 1 mM; Tris 10 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1

Software:

NMRFx - processing

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz
Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

1	MET	ILE	GLY	VAL	LYS	GLU	LEU	ARG	ASP	ALA
2	PHE	ARG	GLU	PHE	ASP	THR	ASN	GLY	ASP	GLY
3	GLU	ILE	SER	THR	SER	GLU	LEU	ARG	GLU	ALA
4	MET	ARG	LYS	LEU	LEU	GLY	HIS	GLN	VAL	GLY
5	HIS	ARG	ASP	ILE	GLU	GLU	ILE	ILE	ARG	ASP
6	VAL	ASP	LEU	ASN	GLY	ASP	GLY	ARG	VAL	ASP
7	PHE	GLU	GLU	PHE	VAL	ARG	MET	MET	SER	ARG

1	MET	ILE	GLY	VAL	LYS	GLU	LEU	ARG	ASP	ALA
2	PHE	ARG	GLU	PHE	ASP	THR	ASN	GLY	ASP	GLY
3	GLU	ILE	SER	THR	SER	GLU	LEU	ARG	GLU	ALA
4	MET	ARG	LYS	LEU	LEU	GLY	HIS	GLN	VAL	GLY
5	HIS	ARG	ASP	ILE	GLU	GLU	ILE	ILE	ARG	ASP
6	VAL	ASP	LEU	ASN	GLY	ASP	GLY	ARG	VAL	ASP
7	PHE	GLU	GLU	PHE	VAL	ARG	MET	MET	SER	ARG

1	MET	ILE	GLY	VAL	LYS	GLU	LEU	ARG	ASP	ALA
2	PHE	ARG	GLU	PHE	ASP	THR	ASN	GLY	ASP	GLY
3	GLU	ILE	SER	THR	SER	GLU	LEU	ARG	GLU	ALA
4	MET	ARG	LYS	LEU	LEU	GLY	HIS	GLN	VAL	GLY
5	HIS	ARG	ASP	ILE	GLU	GLU	ILE	ILE	ARG	ASP
6	VAL	ASP	LEU	ASN	GLY	ASP	GLY	ARG	VAL	ASP
7	PHE	GLU	GLU	PHE	VAL	ARG	MET	MET	SER	ARG