BMRB Entry 51534
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51534
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Title: Sox2-Nterminal(aa1-42)
Deposition date: 2022-07-26 Original release date: 2022-08-26
Authors: Theillet, Francois-Xavier
Citation: Theillet, Francois-Xavier. "Advanced NMR characterization and sensitive detection of isoAs-partate in proteins." .
Assembly members:
entity_1, polymer, 43 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet41b(+)
Entity Sequences (FASTA):
entity_1: GMYNMMETELKPPGPQQTSG
GGGGNSTAAAAGGNQKNSPD
RVK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 115 |
| 15N chemical shifts | 37 |
| 1H chemical shifts | 37 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sox2(aa1-42) | 1 |
Entities:
Entity 1, Sox2(aa1-42) 43 residues - Formula weight is not available
One supplementary Glycine in N-terminal, remaining from Tev-motif (ENLYFQG) after cleavage by Tev-protease.
| 1 | GLY | MET | TYR | ASN | MET | MET | GLU | THR | GLU | LEU | ||||
| 2 | LYS | PRO | PRO | GLY | PRO | GLN | GLN | THR | SER | GLY | ||||
| 3 | GLY | GLY | GLY | GLY | ASN | SER | THR | ALA | ALA | ALA | ||||
| 4 | ALA | GLY | GLY | ASN | GLN | LYS | ASN | SER | PRO | ASP | ||||
| 5 | ARG | VAL | LYS |
Samples:
sample_1: Sox2(aa1-42), [U-98% 13C; U-98% 15N], 0.8 ± 0.1 mM; HEPES 10 mM; sodium chloride 50 mM; DSS 0.5 ± 0.1 mM; protease inhibitor cocktail 2 tablet/100mL; GST-His6-Tev, [U-98% 13C; U-98% 15N], 0.8 ± 0.1 mM; DTT 2 mM; TCEP 5 mM
sample_conditions_1: ionic strength: 0.06 M; pH: 6.8; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.1 - collection, processing
CcpNMR v2.4 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts