BMRB Entry 51540
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51540
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Title: Proteolytic processing induces a conformational switch required for antibacterial toxin delivery PubMed: 36038560
Deposition date: 2022-08-01 Original release date: 2022-08-04
Authors: Bartelli, Nicholas; Passanisi, Victor; Michalska, Karolina; Song, Kiho; Nahn, Dihn; Zhou, Hongjun; Cuthbert, Bonnie; Stols, Lucy; Eschenfeldt, William; Wilson, Nicholas; Basra, Jesse; Cortes, Ricardo; Noorsher, Zainab; Gabraiel, Youssef; Poonen-Honig, Isaac; Seacord, Elizabeth; Goulding, Celia; Low, David; Joachimiak, Andrzej; Dahlquist, Frederick; Hayes, Christopher
Citation: Bartelli, Nicholas; Passanisi, Victor; Michalska, Karolina; Song, Kiho; Nahn, Dihn; Zhou, Hongjun; Cuthbert, Bonnie; Stols, Lucy; Eschenfeldt, William; Wilson, Nicholas; Basra, Jesse; Cortes, Ricardo; Noorsher, Zainab; Gabraiel, Youssef; Poonen-Honig, Isaac; Seacord, Elizabeth; Goulding, Celia; Low, David; Joachimiak, Andrzej; Dahlquist, Frederick; Hayes, Christopher. "Proteolytic processing induces a conformational switch required for antibacterial toxin delivery" Nat. Commun. 13, 5078-5078 (2022).
Assembly members:
entity_1, polymer, 337 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: various
| Data type | Count |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CdiA-CT EC3006 | 1 |
Entities:
Entity 1, CdiA-CT EC3006 337 residues - Formula weight is not available
| 1 | VAL | GLU | ASN | ASN | TYR | LEU | SER | VAL | SER | GLU | ||||
| 2 | LYS | THR | GLU | LEU | GLU | ILE | ALA | LYS | GLN | LYS | ||||
| 3 | LEU | LYS | ASN | SER | LYS | ASP | PRO | ALA | GLU | ARG | ||||
| 4 | GLU | LYS | ALA | GLN | GLN | LYS | TYR | ASP | ALA | LEU | ||||
| 5 | LEU | GLU | LYS | ASP | ILE | SER | SER | ASP | LYS | ALA | ||||
| 6 | VAL | ILE | THR | ALA | CYS | SER | ASN | GLY | GLN | ALA | ||||
| 7 | ALA | SER | ALA | ALA | CYS | ALA | GLY | GLU | ARG | LEU | ||||
| 8 | LYS | VAL | ILE | ALA | ALA | LYS | GLY | GLY | TYR | GLU | ||||
| 9 | THR | GLY | HIS | TYR | ASN | ASN | GLN | VAL | SER | ASP | ||||
| 10 | MET | TYR | PRO | ASP | ALA | TYR | GLY | GLN | ILE | VAL | ||||
| 11 | ASN | LEU | LEU | ASN | ILE | THR | SER | VAL | ASP | ALA | ||||
| 12 | GLN | ASN | GLN | GLN | GLN | VAL | LYS | ASP | ALA | MET | ||||
| 13 | VAL | ASN | TYR | ALA | MET | VAL | GLN | PHE | GLY | VAL | ||||
| 14 | ASP | ARG | ALA | THR | ALA | GLN | ALA | TYR | VAL | GLU | ||||
| 15 | THR | TYR | ASP | GLY | MET | LYS | VAL | VAL | ALA | ALA | ||||
| 16 | SER | MET | ALA | PRO | VAL | ILE | GLY | ALA | ALA | ALA | ||||
| 17 | ALA | SER | LYS | ILE | GLU | VAL | LEU | ALA | GLY | LYS | ||||
| 18 | GLN | ARG | LEU | SER | ASN | SER | PHE | GLU | VAL | SER | ||||
| 19 | SER | LEU | PRO | ASP | ALA | ASN | GLY | LYS | ASN | HIS | ||||
| 20 | ILE | THR | ALA | VAL | LYS | GLY | ASP | ALA | LYS | ILE | ||||
| 21 | PRO | VAL | ASP | LYS | ILE | GLU | LEU | TYR | MET | ARG | ||||
| 22 | GLY | LYS | ALA | SER | GLY | ASP | LEU | ASP | SER | LEU | ||||
| 23 | GLN | ALA | GLU | TYR | ASN | SER | LEU | LYS | ASP | ALA | ||||
| 24 | ARG | ILE | SER | SER | GLN | LYS | GLU | PHE | ALA | LYS | ||||
| 25 | ASP | PRO | ASN | ASN | ALA | LYS | ARG | MET | GLU | VAL | ||||
| 26 | LEU | GLU | LYS | GLN | ILE | HIS | ASN | ILE | GLU | ARG | ||||
| 27 | SER | GLN | ASP | MET | ALA | ARG | VAL | LEU | GLU | GLN | ||||
| 28 | ALA | GLY | ILE | VAL | ASN | THR | ALA | SER | ASN | ASN | ||||
| 29 | SER | MET | ILE | MET | ASP | LYS | LEU | LEU | ASP | SER | ||||
| 30 | ALA | GLN | GLY | ALA | THR | SER | ALA | ASN | ARG | LYS | ||||
| 31 | THR | SER | VAL | VAL | VAL | SER | GLY | PRO | ASN | GLY | ||||
| 32 | ASN | VAL | ARG | ILE | TYR | ALA | THR | TRP | THR | ILE | ||||
| 33 | LEU | PRO | ASP | GLY | THR | LYS | ARG | LEU | SER | THR | ||||
| 34 | VAL | ASN | THR | GLY | THR | PHE | LYS |
Samples:
sample_1: CdiA-CT EC3006, [U-99% 15N], 200 uM; NaPO4 50 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - data analysis
NMR spectrometers:
- Oxford INOVA 600 MHz