BMRB Entry 51544

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51544

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Title:
1H and 15N assignments for [R238A]-NS1B-CTD bound to 16-bp dsRNA
Deposition date:
2022-08-02
Original release date:
2024-08-10
Authors:
Woltz, Ryan; Montelione, Gaetano; Gurla, Swapna; Ma, Li-Chung; John, Rachel
Citation:

Citation: Woltz, Ryan; Schweibenz, Brandon; Tsutakawa, Susan; Zhao, Chen; Ma, LiChung; Shurina, Ben; Hura, Gregory; John, Rachael; Vorobiev, Sergey; Swapna, Gvt; Solotchi, Mihai; Tainer, John; Krug, Robert; Patel, Smita; Montelione, Gaetano. "The NS1 protein of influenza B virus binds 5'-triphosphorylated dsRNA to suppress RIG-I activation and the host antiviral response"  .
PubMed: 38328244

Assembly members:

Assembly members:
entity_1, polymer, 139 residues, 15.69 Da.
entity_2, polymer, 32 residues, 10.25 Da.

Natural source:

Natural source:   Common Name: Influenza B virus   Taxonomy ID: 11520   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betainfluenzavirus Influenza B virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: IEVVLRDMNNKDARQKIKDE VNTQKEGKFRLTIKRDIRNV PSLRVLVNGTFLKHPNGDKS LSTLHRLNAYDQNGGLVAKL VATDDLTVEDEKDGHRILNS LFERFDEGHSKPIRAAETAV GVLSQFGQEHRLSPEEGDN
entity_2: CCAUCCUCUACAGGCGCGCC UGUAGAGGAUGG

Data sets:
Data typeCount
15N chemical shifts129
1H chemical shifts129

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2RNA2

Entities:

Entity 1, protein 139 residues - 15.69 Da.

1   ILEGLUVALVALLEUARGASPMETASNASN
2   LYSASPALAARGGLNLYSILELYSASPGLU
3   VALASNTHRGLNLYSGLUGLYLYSPHEARG
4   LEUTHRILELYSARGASPILEARGASNVAL
5   PROSERLEUARGVALLEUVALASNGLYTHR
6   PHELEULYSHISPROASNGLYASPLYSSER
7   LEUSERTHRLEUHISARGLEUASNALATYR
8   ASPGLNASNGLYGLYLEUVALALALYSLEU
9   VALALATHRASPASPLEUTHRVALGLUASP
10   GLULYSASPGLYHISARGILELEUASNSER
11   LEUPHEGLUARGPHEASPGLUGLYHISSER
12   LYSPROILEARGALAALAGLUTHRALAVAL
13   GLYVALLEUSERGLNPHEGLYGLNGLUHIS
14   ARGLEUSERPROGLUGLUGLYASPASN

Entity 2, RNA 32 residues - 10.25 Da.

1   CCAUCCUCUA
2   CAGGCGCGCC
3   UGUAGAGGAU
4   GG

Related Database Links:

UNP P03502

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks