BMRB Entry 51598
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51598
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Title: SNAP25a (Rattus norvegicus) PubMed: 37003471
Deposition date: 2022-08-24 Original release date: 2022-08-31
Authors: Stief, Tobias; Lakomek, Nils-Alexander
Citation: Stief, Tobias; Gremer, Lothar; Pribicevic, Sonja; Espinueva, Delane; Vormann, Katharina; Biehl, Ralf; Jahn, Reinhard; Perez-Lara, Angel; Lakomek, Nils-Alexander. "Intrinsic Disorder of the Neuronal SNARE Protein SNAP25a in its Pre-fusion Conformation" J. Mol. Biol. 435, 168069-168069 (2023).
Assembly members:
entity_1, polymer, 206 residues, 23272 Da.
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a(+)
Entity Sequences (FASTA):
entity_1: MAEDADMRNELEEMQRRADQ
LADESLESTRRMLQLVEESK
DAGIRTLVMLDEQGEQLDRV
EEGMNHINQDMKEAEKNLKD
LGKSSGLFISPSNKLKSSDA
YKKAWGNNQDGVVASQPARV
VDEREQMAISGGFIRRVTND
ARENEMDENLEQVSGIIGNL
RHMALDMGNEIDTQNRQIDR
IMEKADSNKTRIDEANQRAT
KMLGSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 595 |
| 15N chemical shifts | 204 |
| 1H chemical shifts | 204 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SNAP25a | 1 |
Entities:
Entity 1, SNAP25a 206 residues - 23272 Da.
| 1 | MET | ALA | GLU | ASP | ALA | ASP | MET | ARG | ASN | GLU | ||||
| 2 | LEU | GLU | GLU | MET | GLN | ARG | ARG | ALA | ASP | GLN | ||||
| 3 | LEU | ALA | ASP | GLU | SER | LEU | GLU | SER | THR | ARG | ||||
| 4 | ARG | MET | LEU | GLN | LEU | VAL | GLU | GLU | SER | LYS | ||||
| 5 | ASP | ALA | GLY | ILE | ARG | THR | LEU | VAL | MET | LEU | ||||
| 6 | ASP | GLU | GLN | GLY | GLU | GLN | LEU | ASP | ARG | VAL | ||||
| 7 | GLU | GLU | GLY | MET | ASN | HIS | ILE | ASN | GLN | ASP | ||||
| 8 | MET | LYS | GLU | ALA | GLU | LYS | ASN | LEU | LYS | ASP | ||||
| 9 | LEU | GLY | LYS | SER | SER | GLY | LEU | PHE | ILE | SER | ||||
| 10 | PRO | SER | ASN | LYS | LEU | LYS | SER | SER | ASP | ALA | ||||
| 11 | TYR | LYS | LYS | ALA | TRP | GLY | ASN | ASN | GLN | ASP | ||||
| 12 | GLY | VAL | VAL | ALA | SER | GLN | PRO | ALA | ARG | VAL | ||||
| 13 | VAL | ASP | GLU | ARG | GLU | GLN | MET | ALA | ILE | SER | ||||
| 14 | GLY | GLY | PHE | ILE | ARG | ARG | VAL | THR | ASN | ASP | ||||
| 15 | ALA | ARG | GLU | ASN | GLU | MET | ASP | GLU | ASN | LEU | ||||
| 16 | GLU | GLN | VAL | SER | GLY | ILE | ILE | GLY | ASN | LEU | ||||
| 17 | ARG | HIS | MET | ALA | LEU | ASP | MET | GLY | ASN | GLU | ||||
| 18 | ILE | ASP | THR | GLN | ASN | ARG | GLN | ILE | ASP | ARG | ||||
| 19 | ILE | MET | GLU | LYS | ALA | ASP | SER | ASN | LYS | THR | ||||
| 20 | ARG | ILE | ASP | GLU | ALA | ASN | GLN | ARG | ALA | THR | ||||
| 21 | LYS | MET | LEU | GLY | SER | GLY |
Samples:
sample_1: SNAP25a, [U-99% 13C; U-99% 15N], 150 uM; sodium phosphate 20 mM; sodium chloride 150 mM; TCEP 0.1 mM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 288.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.5 - processing
CcpNMR v3 - peak picking
NMR spectrometers:
- Bruker AVANCE III 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts