BMRB Entry 51606
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51606
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Title: KH1-2 Fragile X mental retardation protein D331-396 PubMed: 37426752
Deposition date: 2022-08-31 Original release date: 2023-12-19
Authors: Chaves Arquero, Belen; M. Collins, Katherine; Abis, Giancarlo; Ramos, Andres
Citation: Chaves Arquero, Belen; M. Collins, Katherine; Abis, Giancarlo; Kelly, Geoff; Christodoulou, Evangelios; Taylor, Ian; Ramos, Andres. "Affinity-enhanced RNA-binding domains as tools to understand RNA recognition" Cell Rep. Methods 3, 100508-100508 (2023).
Assembly members:
entity_1, polymer, 144 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-47b
Entity Sequences (FASTA):
entity_1: ASRFHEQFIVREDLMGLAIG
THGANIQQARKVPGVTAIDL
DEDTCTFHIYGEDQDAVKKA
RSFLEFAEDVIQVPRNLVGK
VIGKNGKLIQEIVDKSGVVR
VRIEAENEKNVPQEEGMVPF
VFVGTKDSIANATVLLDYHL
NYLK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 174 |
| 15N chemical shifts | 94 |
| 1H chemical shifts | 95 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Didomain KH1KH2 | 1 |
Entities:
Entity 1, Didomain KH1KH2 144 residues - Formula weight is not available
| 1 | ALA | SER | ARG | PHE | HIS | GLU | GLN | PHE | ILE | VAL | ||||
| 2 | ARG | GLU | ASP | LEU | MET | GLY | LEU | ALA | ILE | GLY | ||||
| 3 | THR | HIS | GLY | ALA | ASN | ILE | GLN | GLN | ALA | ARG | ||||
| 4 | LYS | VAL | PRO | GLY | VAL | THR | ALA | ILE | ASP | LEU | ||||
| 5 | ASP | GLU | ASP | THR | CYS | THR | PHE | HIS | ILE | TYR | ||||
| 6 | GLY | GLU | ASP | GLN | ASP | ALA | VAL | LYS | LYS | ALA | ||||
| 7 | ARG | SER | PHE | LEU | GLU | PHE | ALA | GLU | ASP | VAL | ||||
| 8 | ILE | GLN | VAL | PRO | ARG | ASN | LEU | VAL | GLY | LYS | ||||
| 9 | VAL | ILE | GLY | LYS | ASN | GLY | LYS | LEU | ILE | GLN | ||||
| 10 | GLU | ILE | VAL | ASP | LYS | SER | GLY | VAL | VAL | ARG | ||||
| 11 | VAL | ARG | ILE | GLU | ALA | GLU | ASN | GLU | LYS | ASN | ||||
| 12 | VAL | PRO | GLN | GLU | GLU | GLY | MET | VAL | PRO | PHE | ||||
| 13 | VAL | PHE | VAL | GLY | THR | LYS | ASP | SER | ILE | ALA | ||||
| 14 | ASN | ALA | THR | VAL | LEU | LEU | ASP | TYR | HIS | LEU | ||||
| 15 | ASN | TYR | LEU | LYS |
Samples:
sample_1: Fragile X mental retardation protein D331-396, [U-13C; U-15N; U-2H], 200 uM
sample_conditions_1: pH: 6.9; temperature: 301.2 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts