BMRB Entry 51611
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51611
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Title: 1H, 13C and 15N Backbone Chemical Shift Assignments of NUT7 PubMed: 36522330
Deposition date: 2022-09-01 Original release date: 2022-11-29
Authors: Salvi, Nicola; Ibrahim, Ziad; Blackledge, Martin; Panne, Daniel
Citation: Ibrahim, Ziad; Wang, Tao; Destaing, Olivier; Salvi, Nicola; Hoghoughi, Naghmeh; Chabert, Clovis; Rusu, Alexandra; Gao, Jinjun; Feletto, Leonardo; Reynoird, Nicolas; Schalch, Thomas; Zhao, Yingming; Blackledge, Martin; Khochbin, Saadi; Panne, Daniel. "Structural insights into p300 regulation and acetylation-dependent genome organisation" Nat. Commun. 13, 7759-7759 (2022).
Assembly members:
entity_1, polymer, 50 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: -
Entity Sequences (FASTA):
entity_1: GAMAWPLALIEELEQEEGLT
LAQLVQKRLMALEEEEDAEA
PPSFSGAQLD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 79 |
| 15N chemical shifts | 39 |
| 1H chemical shifts | 39 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NUT7 | 1 |
Entities:
Entity 1, NUT7 50 residues - Formula weight is not available
| 1 | GLY | ALA | MET | ALA | TRP | PRO | LEU | ALA | LEU | ILE | |
| 2 | GLU | GLU | LEU | GLU | GLN | GLU | GLU | GLY | LEU | THR | |
| 3 | LEU | ALA | GLN | LEU | VAL | GLN | LYS | ARG | LEU | MET | |
| 4 | ALA | LEU | GLU | GLU | GLU | GLU | ASP | ALA | GLU | ALA | |
| 5 | PRO | PRO | SER | PHE | SER | GLY | ALA | GLN | LEU | ASP |
Samples:
sample_1: NUT7, [U-13C; U-15N], 500 uM; Tris 20 mM; NaCl 300 mM; DTT 1 mM; ZnSO4 5 uM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts