BMRB Entry 51612
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51612
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Uracil-DNA glycosylase efficiency is modulated by substrate rigidity PubMed: 36890276
Deposition date: 2022-09-02 Original release date: 2023-03-14
Authors: Orndorff, Paul; Poddar, Souvik; Owens, Aerial; Kumari, Nikita; Ugaz, Bryan; Amin, Samrat; Van Horn, Wade; van der Vaart, Arjan; Levitus, Marcia
Citation: Orndorff, Paul; Poddar, Souvik; Owens, Aerial; Kumari, Nikita; Ugaz, Bryan; Amin, Samrat; Van Horn, Wade; van der Vaart, Arjan; Levitus, Marcia. "Uracil-DNA glycosylase efficiency is modulated by substrate rigidity" Sci. Rep. 13, 3915-3915 (2023).
Assembly members:
entity_1, polymer, 13 residues, Formula weight is not available
entity_2, polymer, 13 residues, Formula weight is not available
entity_3, polymer, 13 residues, Formula weight is not available
entity_4, polymer, 13 residues, Formula weight is not available
entity_5, polymer, 13 residues, Formula weight is not available
entity_6, polymer, 13 residues, Formula weight is not available
entity_7, polymer, 13 residues, Formula weight is not available
entity_8, polymer, 13 residues, Formula weight is not available
entity_9, polymer, 13 residues, Formula weight is not available
entity_10, polymer, 13 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
entity_1: GTGAAAUTGTTAC
entity_2: GTAACAATTTCAC
entity_3: GTGAATUAGTTAC
entity_4: GTAACTAATTCAC
entity_5: GTGAAAUAGTTAC
entity_6: GTAACTATTTCAC
entity_7: GTGAATUTGTTAC
entity_8: GTAACAAATTCAC
entity_9: GTGCAAUTATGAC
entity_10: GTCATAATTGCAC
| Data type | Count |
| H exchange rates | 38 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 4ATF | 1 |
| 2 | 4ATR | 2 |
| 3 | 4TAF | 3 |
| 4 | 4TAR | 4 |
| 5 | 4AAF | 5 |
| 6 | 4AAR | 6 |
| 7 | 4TTF | 7 |
| 8 | 4TTR | 8 |
| 9 | 1ATF | 9 |
| 10 | 1ATR | 10 |
Entities:
Entity 1, 4ATF 13 residues - Formula weight is not available
complement strand pairs A with U
| 1 | DG | DT | DG | DA | DA | DA | DU | DT | DG | DT | ||||
| 2 | DT | DA | DC |
Entity 2, 4ATR 13 residues - Formula weight is not available
complement strand pairs A with U
| 1 | DG | DT | DA | DA | DC | DA | DA | DT | DT | DT | ||||
| 2 | DC | DA | DC |
Entity 3, 4TAF 13 residues - Formula weight is not available
complement strand pairs A with U
| 1 | DG | DT | DG | DA | DA | DT | DU | DA | DG | DT | ||||
| 2 | DT | DA | DC |
Entity 4, 4TAR 13 residues - Formula weight is not available
complement strand pairs A with U
| 1 | DG | DT | DA | DA | DC | DT | DA | DA | DT | DT | ||||
| 2 | DC | DA | DC |
Entity 5, 4AAF 13 residues - Formula weight is not available
complement strand pairs A with U
| 1 | DG | DT | DG | DA | DA | DA | DU | DA | DG | DT | ||||
| 2 | DT | DA | DC |
Entity 6, 4AAR 13 residues - Formula weight is not available
complement strand pairs A with U
| 1 | DG | DT | DA | DA | DC | DT | DA | DT | DT | DT | ||||
| 2 | DC | DA | DC |
Entity 7, 4TTF 13 residues - Formula weight is not available
complement strand pairs A with U
| 1 | DG | DT | DG | DA | DA | DT | DU | DT | DG | DT | ||||
| 2 | DT | DA | DC |
Entity 8, 4TTR 13 residues - Formula weight is not available
complement strand pairs A with U
| 1 | DG | DT | DA | DA | DC | DA | DA | DA | DT | DT | ||||
| 2 | DC | DA | DC |
Entity 9, 1ATF 13 residues - Formula weight is not available
complement strand pairs A with U
| 1 | DG | DT | DG | DC | DA | DA | DU | DT | DA | DT | ||||
| 2 | DG | DA | DC |
Entity 10, 1ATR 13 residues - Formula weight is not available
complement strand pairs A with U
| 1 | DG | DT | DC | DA | DT | DA | DA | DT | DT | DG | ||||
| 2 | DC | DA | DC |
Samples:
sample_1: 4ATF 3 mM; 4ATR 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; EDTA 50 uM
sample_2: 4TAF 3 mM; 4TAR 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; EDTA 50 uM
sample_3: 4AAF 3 mM; 4AAR 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; EDTA 50 uM
sample_4: 4TTF 3 mM; 4TTR 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; EDTA 50 uM
sample_5: 1ATF 3 mM; 1ATR 3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; EDTA 50 uM
sample_conditions_1: pH: 7.0; temperature: 293.15 K
sample_conditions_2: pH: 7.0; temperature: 293.15 K
sample_conditions_3: pH: 7.0; temperature: 293.15 K
sample_conditions_4: pH: 7.0; temperature: 293.15 K
sample_conditions_5: pH: 7.0; temperature: 293.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_3 | isotropic | sample_conditions_3 |
| 2D 1H-1H NOESY | sample_4 | isotropic | sample_conditions_4 |
| 2D 1H-1H NOESY | sample_5 | isotropic | sample_conditions_5 |
| T1/R1 relaxation | sample_1 | isotropic | sample_conditions_1 |
| T1/R1 relaxation | sample_2 | isotropic | sample_conditions_2 |
| T1/R1 relaxation | sample_3 | isotropic | sample_conditions_3 |
| T1/R1 relaxation | sample_4 | isotropic | sample_conditions_4 |
| T1/R1 relaxation | sample_5 | isotropic | sample_conditions_5 |
Software:
TOPSPIN v4.1 - chemical shift assignment, collection, processing
MATLAB v2019b - data analysis, processing
NMR spectrometers:
- Bruker AVANCE III 850 MHz
- Bruker AVANCE III 600 MHz