BMRB Entry 51620

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51620

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Title:
NMR assignment of NTD (44 - 180) of Nucleocapsid Protein of SARS-CoV-2
Deposition date:
2022-09-09
Original release date:
2022-10-14
Authors:
Schiavina, Marco; Tagliaferro, Giuseppe; Pontoriero, Letizia; Pierattelli, Roberta; Felli, Isabella Caterina
Citation:

Citation: Schiavina, Marco; Pontoriero, Letizia; Tagliaferro, Giuseppe; Pierattelli, Roberta; Felli, Isabella Caterina. "The Role of Disordered Regions in Orchestrating the Properties of Multidomain Proteins: The SARS-CoV-2 Nucleocapsid Protein and Its Interaction with Enoxaparin"  Biomolecules 12, 1302-1302 (2022).
PubMed: 36139141

Assembly members:

Assembly members:
entity_1, polymer, 137 residues, 14850 Da.

Natural source:

Natural source:   Common Name: SARS-CoV-2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GLPNNTASWFTALTQHGKED LKFPRGQGVPINTNSSPDDQ IGYYRRATRRIRGGDGKMKD LSPRWYFYYLGTGPEAGLPY GANKDGIIWVATEGALNTPK DHIGTRNPANNAAIVLQLPQ GTTLPKGFYAEGSRGGS

Data sets:
Data typeCount
13C chemical shifts563
15N chemical shifts145
1H chemical shifts911

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NTD1

Entities:

Entity 1, NTD 137 residues - 14850 Da.

1   GLYLEUPROASNASNTHRALASERTRPPHE
2   THRALALEUTHRGLNHISGLYLYSGLUASP
3   LEULYSPHEPROARGGLYGLNGLYVALPRO
4   ILEASNTHRASNSERSERPROASPASPGLN
5   ILEGLYTYRTYRARGARGALATHRARGARG
6   ILEARGGLYGLYASPGLYLYSMETLYSASP
7   LEUSERPROARGTRPTYRPHETYRTYRLEU
8   GLYTHRGLYPROGLUALAGLYLEUPROTYR
9   GLYALAASNLYSASPGLYILEILETRPVAL
10   ALATHRGLUGLYALALEUASNTHRPROLYS
11   ASPHISILEGLYTHRARGASNPROALAASN
12   ASNALAALAILEVALLEUGLNLEUPROGLN
13   GLYTHRTHRLEUPROLYSGLYPHETYRALA
14   GLUGLYSERARGGLYGLYSER

Related Database Links:

UNP P0DTC9
AlphaFold P0DTC9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks