BMRB Entry 51620
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51620
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Title: NMR assignment of NTD (44 - 180) of Nucleocapsid Protein of SARS-CoV-2 PubMed: 36139141
Deposition date: 2022-09-09 Original release date: 2022-10-14
Authors: Schiavina, Marco; Tagliaferro, Giuseppe; Pontoriero, Letizia; Pierattelli, Roberta; Felli, Isabella Caterina
Citation: Schiavina, Marco; Pontoriero, Letizia; Tagliaferro, Giuseppe; Pierattelli, Roberta; Felli, Isabella Caterina. "The Role of Disordered Regions in Orchestrating the Properties of Multidomain Proteins: The SARS-CoV-2 Nucleocapsid Protein and Its Interaction with Enoxaparin" Biomolecules 12, 1302-1302 (2022).
Assembly members:
entity_1, polymer, 137 residues, 14850 Da.
Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a(+)
Entity Sequences (FASTA):
entity_1: GLPNNTASWFTALTQHGKED
LKFPRGQGVPINTNSSPDDQ
IGYYRRATRRIRGGDGKMKD
LSPRWYFYYLGTGPEAGLPY
GANKDGIIWVATEGALNTPK
DHIGTRNPANNAAIVLQLPQ
GTTLPKGFYAEGSRGGS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 423 |
| 15N chemical shifts | 145 |
| 1H chemical shifts | 134 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NTD | 1 |
Entities:
Entity 1, NTD 137 residues - 14850 Da.
| 1 | GLY | LEU | PRO | ASN | ASN | THR | ALA | SER | TRP | PHE | ||||
| 2 | THR | ALA | LEU | THR | GLN | HIS | GLY | LYS | GLU | ASP | ||||
| 3 | LEU | LYS | PHE | PRO | ARG | GLY | GLN | GLY | VAL | PRO | ||||
| 4 | ILE | ASN | THR | ASN | SER | SER | PRO | ASP | ASP | GLN | ||||
| 5 | ILE | GLY | TYR | TYR | ARG | ARG | ALA | THR | ARG | ARG | ||||
| 6 | ILE | ARG | GLY | GLY | ASP | GLY | LYS | MET | LYS | ASP | ||||
| 7 | LEU | SER | PRO | ARG | TRP | TYR | PHE | TYR | TYR | LEU | ||||
| 8 | GLY | THR | GLY | PRO | GLU | ALA | GLY | LEU | PRO | TYR | ||||
| 9 | GLY | ALA | ASN | LYS | ASP | GLY | ILE | ILE | TRP | VAL | ||||
| 10 | ALA | THR | GLU | GLY | ALA | LEU | ASN | THR | PRO | LYS | ||||
| 11 | ASP | HIS | ILE | GLY | THR | ARG | ASN | PRO | ALA | ASN | ||||
| 12 | ASN | ALA | ALA | ILE | VAL | LEU | GLN | LEU | PRO | GLN | ||||
| 13 | GLY | THR | THR | LEU | PRO | LYS | GLY | PHE | TYR | ALA | ||||
| 14 | GLU | GLY | SER | ARG | GLY | GLY | SER |
Samples:
sample_1: NTD (44-180) of N protein from SARS-CoV-2, [U-100% 13C; U-100% 15N], 450 uM; KPi 25 mM; KCl 150 mM; NaN3 0.03%
sample_conditions_1: ionic strength: 170 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C CACO | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C CBCACO | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C CON | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 13C (H)CBCACON | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 950 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts