BMRB Entry 51638
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51638
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Title: Backbone assignment of cJun TAD 1-151 PubMed: 36253406
Deposition date: 2022-09-22 Original release date: 2022-09-26
Authors: Waudby, Christopher; Alvarez-Teijeiro, Saul; Ruiz, E.; Suppinger, Simon; Pinotsis, Nikos; Brown, Paul; Behrens, Axel; Christodoulou, John; Mylona, Anastasia
Citation: Waudby, Christopher; Alvarez-Teijeiro, Saul; Ruiz, E.; Suppinger, Simon; Pinotsis, Nikos; Brown, Paul; Behrens, Axel; Christodoulou, John; Mylona, Anastasia. "An intrinsic temporal order of c-JUN N-terminal phosphorylation regulates its activity by orchestrating co-factor recruitment" Nat. Commun. 13, 6133-6133 (2022).
Assembly members:
entity_1, polymer, 154 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-41a
Entity Sequences (FASTA):
entity_1: GPGSTAKMETTFYDDALNAS
FLPSESGPYGYSNPKILKQS
MTLNLADPVGSLKPHLRAKN
SDLLTSPDVGLLKLASPELE
RLIIQSSNGHITTTPTPTQF
LCPKNVTDEQEGFAEGFVRA
LAELHSQNTLPSVTSAAQPV
NGAGMVAPAVASVA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 438 |
| 15N chemical shifts | 135 |
| 1H chemical shifts | 135 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cJun | 1 |
Entities:
Entity 1, cJun 154 residues - Formula weight is not available
Residues 1-4 represent a TEV cleavage site
| 1 | GLY | PRO | GLY | SER | THR | ALA | LYS | MET | GLU | THR | ||||
| 2 | THR | PHE | TYR | ASP | ASP | ALA | LEU | ASN | ALA | SER | ||||
| 3 | PHE | LEU | PRO | SER | GLU | SER | GLY | PRO | TYR | GLY | ||||
| 4 | TYR | SER | ASN | PRO | LYS | ILE | LEU | LYS | GLN | SER | ||||
| 5 | MET | THR | LEU | ASN | LEU | ALA | ASP | PRO | VAL | GLY | ||||
| 6 | SER | LEU | LYS | PRO | HIS | LEU | ARG | ALA | LYS | ASN | ||||
| 7 | SER | ASP | LEU | LEU | THR | SER | PRO | ASP | VAL | GLY | ||||
| 8 | LEU | LEU | LYS | LEU | ALA | SER | PRO | GLU | LEU | GLU | ||||
| 9 | ARG | LEU | ILE | ILE | GLN | SER | SER | ASN | GLY | HIS | ||||
| 10 | ILE | THR | THR | THR | PRO | THR | PRO | THR | GLN | PHE | ||||
| 11 | LEU | CYS | PRO | LYS | ASN | VAL | THR | ASP | GLU | GLN | ||||
| 12 | GLU | GLY | PHE | ALA | GLU | GLY | PHE | VAL | ARG | ALA | ||||
| 13 | LEU | ALA | GLU | LEU | HIS | SER | GLN | ASN | THR | LEU | ||||
| 14 | PRO | SER | VAL | THR | SER | ALA | ALA | GLN | PRO | VAL | ||||
| 15 | ASN | GLY | ALA | GLY | MET | VAL | ALA | PRO | ALA | VAL | ||||
| 16 | ALA | SER | VAL | ALA |
Samples:
sample_1: cJun TAD, [U-100% 13C; U-100% 15N], 150 uM; K2HPO4 10 mM; NaCl 150 mM; MgCl2 5 mM; DSS 0.01 w/v
sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.4 - chemical shift assignment, peak picking
TOPSPIN v3.5pl6 - collection
NMRPipe v10.9 - processing
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts