BMRB Entry 51650
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51650
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Title: Ded1p N-terminal IDR
Deposition date: 2022-10-04 Original release date: 2022-10-10
Authors: Schneider, Jakob
Citation: schneider, Jakob. "Ded1p N-terminal IDR" to be published ., .-..
Assembly members:
entity_1, polymer, 81 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11
Entity Sequences (FASTA):
entity_1: MAELSEQVQNLSINDNNENG
YVPPHLRGKPRSARNNSSNY
NNNNGGYNGGRGGGSFFSNN
RRGGYGNGGFFGGNNGGSRS
N
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 193 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 69 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ded1p N-terminal IDR | 1 |
Entities:
Entity 1, Ded1p N-terminal IDR 81 residues - Formula weight is not available
| 1 | MET | ALA | GLU | LEU | SER | GLU | GLN | VAL | GLN | ASN | ||||
| 2 | LEU | SER | ILE | ASN | ASP | ASN | ASN | GLU | ASN | GLY | ||||
| 3 | TYR | VAL | PRO | PRO | HIS | LEU | ARG | GLY | LYS | PRO | ||||
| 4 | ARG | SER | ALA | ARG | ASN | ASN | SER | SER | ASN | TYR | ||||
| 5 | ASN | ASN | ASN | ASN | GLY | GLY | TYR | ASN | GLY | GLY | ||||
| 6 | ARG | GLY | GLY | GLY | SER | PHE | PHE | SER | ASN | ASN | ||||
| 7 | ARG | ARG | GLY | GLY | TYR | GLY | ASN | GLY | GLY | PHE | ||||
| 8 | PHE | GLY | GLY | ASN | ASN | GLY | GLY | SER | ARG | SER | ||||
| 9 | ASN |
Samples:
sample_1: Ded1p N-terminal IDR, [U-13C; U-15N], 300 uM; sodium chloride 125 mM; HEPES 20 mM; DTT 1 mM; sodium azide 0.03%
sample_conditions_1: ionic strength: 0.125 M; pH: 7.3; pressure: 1 atm; temperature: 277 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts