BMRB Entry 51654
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51654
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Title: Backbone N and HN Chemical Shift Assignments for RBPMS-A (1-122) PubMed: 36408906
Deposition date: 2022-10-06 Original release date: 2022-10-10
Authors: Barnhart, Michael; Yang, Yi; Nakagaki-Silva, Erick; Hammond, Thomas; Pizzinga, Mariavittoria; Gooding, Clare; Stott, Katherine; Smith, Christopher
Citation: Barnhart, Michael; Yang, Yi; Nakagaki-Silva, Erick; Hammond, Thomas; Pizzinga, Mariavittoria; Gooding, Clare; Stott, Katherine; Smith, Christopher. "Phosphorylation of the smooth muscle master splicing regulator RBPMS regulates its splicing activity" Nucleic Acids Res. 50, 11895-11915 (2022).
Assembly members:
entity_1, polymer, 122 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b
Entity Sequences (FASTA):
entity_1: MNGGGKAEKENTPSEANLQE
EEVRTLFVSGLPLDIKPREL
YLLFRPFKGYEGSLIKLTSK
QPVGFVSFDSRSEAEAAKNA
LNGIRFDPEIPQTLRLEFAK
ANTKMAKNKLVGTPNPSTPL
PN
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 94 |
| 1H chemical shifts | 94 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RBPMS-A | 1 |
Entities:
Entity 1, RBPMS-A 122 residues - Formula weight is not available
| 1 | MET | ASN | GLY | GLY | GLY | LYS | ALA | GLU | LYS | GLU | ||||
| 2 | ASN | THR | PRO | SER | GLU | ALA | ASN | LEU | GLN | GLU | ||||
| 3 | GLU | GLU | VAL | ARG | THR | LEU | PHE | VAL | SER | GLY | ||||
| 4 | LEU | PRO | LEU | ASP | ILE | LYS | PRO | ARG | GLU | LEU | ||||
| 5 | TYR | LEU | LEU | PHE | ARG | PRO | PHE | LYS | GLY | TYR | ||||
| 6 | GLU | GLY | SER | LEU | ILE | LYS | LEU | THR | SER | LYS | ||||
| 7 | GLN | PRO | VAL | GLY | PHE | VAL | SER | PHE | ASP | SER | ||||
| 8 | ARG | SER | GLU | ALA | GLU | ALA | ALA | LYS | ASN | ALA | ||||
| 9 | LEU | ASN | GLY | ILE | ARG | PHE | ASP | PRO | GLU | ILE | ||||
| 10 | PRO | GLN | THR | LEU | ARG | LEU | GLU | PHE | ALA | LYS | ||||
| 11 | ALA | ASN | THR | LYS | MET | ALA | LYS | ASN | LYS | LEU | ||||
| 12 | VAL | GLY | THR | PRO | ASN | PRO | SER | THR | PRO | LEU | ||||
| 13 | PRO | ASN |
Samples:
sample_1: RBPMS-A, [U-98% 13C; U-98% 15N], 0.5 mM; HEPES 10 mM; potassium chloride 50 mM
sample_conditions_1: ionic strength: 60 mM; pH: 6.8; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.4 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts