BMRB Entry 51655

Name: UBE3A isoform 2 AZUL
Published: 2022-11-07

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PDB ID: 8ept
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51655
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

UBE3A isoform 2 AZUL

Deposition date:

2022-10-06

Original release date:

2022-11-07

Authors:

Bregnard, Thomas; Bezsonova, Irina

Citation:

Citation: Bregnard, Thomas; Fairchild, Daniel; Chen, Xiang; Erlandsen, Heidi; Walters, Kylie; Korzhnev, Dmitry; Bezsonova, Irina. "Differences in structure, dynamics and Zn-coordination between isoforms of human ubiquitin ligase UBE3A" .

Assembly members:

Assembly members:
entity_1, polymer, . residues, 10201.56 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSHMEKLHQCYWKSGEPQSD DIEASRMKRAAAKHLIERYY HQLTEGCGNEACTNEFCASC PTFLRMDNNAAAIKALELYK INAKLCDPHP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	368
15N chemical shifts	92
1H chemical shifts	548

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	polypeptide chain	1
2	zinc	2

Entities:

Entity 1, polypeptide chain - 10201.56 Da.

Residues 1-3 are left over from tag cleavage. Residues 4-90 comprise the extended AZUL domain of UBE3A isoform 2.

1	GLY	SER	HIS	MET	GLU	LYS	LEU	HIS	GLN	CYS
2	TYR	TRP	LYS	SER	GLY	GLU	PRO	GLN	SER	ASP
3	ASP	ILE	GLU	ALA	SER	ARG	MET	LYS	ARG	ALA
4	ALA	ALA	LYS	HIS	LEU	ILE	GLU	ARG	TYR	TYR
5	HIS	GLN	LEU	THR	GLU	GLY	CYS	GLY	ASN	GLU
6	ALA	CYS	THR	ASN	GLU	PHE	CYS	ALA	SER	CYS
7	PRO	THR	PHE	LEU	ARG	MET	ASP	ASN	ASN	ALA
8	ALA	ALA	ILE	LYS	ALA	LEU	GLU	LEU	TYR	LYS
9	ILE	ASN	ALA	LYS	LEU	CYS	ASP	PRO	HIS	PRO

Entity 2, zinc - Zn - 65.409 Da.

1

ZN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 51655

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	GLY	SER	HIS	MET	GLU	LYS	LEU	HIS	GLN	CYS
2	TYR	TRP	LYS	SER	GLY	GLU	PRO	GLN	SER	ASP
3	ASP	ILE	GLU	ALA	SER	ARG	MET	LYS	ARG	ALA
4	ALA	ALA	LYS	HIS	LEU	ILE	GLU	ARG	TYR	TYR
5	HIS	GLN	LEU	THR	GLU	GLY	CYS	GLY	ASN	GLU
6	ALA	CYS	THR	ASN	GLU	PHE	CYS	ALA	SER	CYS
7	PRO	THR	PHE	LEU	ARG	MET	ASP	ASN	ASN	ALA
8	ALA	ALA	ILE	LYS	ALA	LEU	GLU	LEU	TYR	LYS
9	ILE	ASN	ALA	LYS	LEU	CYS	ASP	PRO	HIS	PRO

1	GLY	SER	HIS	MET	GLU	LYS	LEU	HIS	GLN	CYS
2	TYR	TRP	LYS	SER	GLY	GLU	PRO	GLN	SER	ASP
3	ASP	ILE	GLU	ALA	SER	ARG	MET	LYS	ARG	ALA
4	ALA	ALA	LYS	HIS	LEU	ILE	GLU	ARG	TYR	TYR
5	HIS	GLN	LEU	THR	GLU	GLY	CYS	GLY	ASN	GLU
6	ALA	CYS	THR	ASN	GLU	PHE	CYS	ALA	SER	CYS
7	PRO	THR	PHE	LEU	ARG	MET	ASP	ASN	ASN	ALA
8	ALA	ALA	ILE	LYS	ALA	LEU	GLU	LEU	TYR	LYS
9	ILE	ASN	ALA	LYS	LEU	CYS	ASP	PRO	HIS	PRO

1	GLY	SER	HIS	MET	GLU	LYS	LEU	HIS	GLN	CYS
2	TYR	TRP	LYS	SER	GLY	GLU	PRO	GLN	SER	ASP
3	ASP	ILE	GLU	ALA	SER	ARG	MET	LYS	ARG	ALA
4	ALA	ALA	LYS	HIS	LEU	ILE	GLU	ARG	TYR	TYR
5	HIS	GLN	LEU	THR	GLU	GLY	CYS	GLY	ASN	GLU
6	ALA	CYS	THR	ASN	GLU	PHE	CYS	ALA	SER	CYS
7	PRO	THR	PHE	LEU	ARG	MET	ASP	ASN	ASN	ALA
8	ALA	ALA	ILE	LYS	ALA	LEU	GLU	LEU	TYR	LYS
9	ILE	ASN	ALA	LYS	LEU	CYS	ASP	PRO	HIS	PRO