BMRB Entry 51687
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51687
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Title: Backbone and CB chemical shifts of HPy1 repetitive unit from Argiope argentata PubMed: 36802452
Deposition date: 2022-11-05 Original release date: 2023-03-14
Authors: Simmons, Jeffrey; Rainey, Jan
Citation: Simmons, Jeffrey; Gasmi-Seabrook, Genevieve; Rainey, Jan. "Structural Features, Intrinsic Disorder, and Modularity of a Pyriform Spidroin 1 Core Repetitive Domain" Biochem. Cell Biol. 101, 271-283 (2023).
Assembly members:
entity_1, polymer, 243 residues, 24509.6595 Da.
Natural source: Common Name: Argiope argentata Taxonomy ID: 233271 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Argiope argentata
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32
Entity Sequences (FASTA):
entity_1: MGHHHHHHSSAQENSFTQSS
VAQQSAVAQQSSVSQQSSAA
QQSSVAQSQQTSYSAATNAG
SSVSQSQAIVSSAPVYFNSQ
TLTNNLASSLQSLNALNYVS
NGQLSSSDVASTVARAVAQS
LGLSQGSVQNIMSQQLSSIG
SGASTSSLSQAIANAVSSAV
QGSQAAAPGQEQSIAQRVNS
AISSAFAQLISQRTAPAPAP
RPRPAPLPAPAPRPRPAPAP
RPAPVYAPAPVASQFQASAS
SQS
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 569 |
15N chemical shifts | 190 |
1H chemical shifts | 185 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | HPy1 v2 | 1 |
Entities:
Entity 1, HPy1 v2 243 residues - 24509.6595 Da.
1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | SER | SER | ||||
2 | ALA | GLN | GLU | ASN | SER | PHE | THR | GLN | SER | SER | ||||
3 | VAL | ALA | GLN | GLN | SER | ALA | VAL | ALA | GLN | GLN | ||||
4 | SER | SER | VAL | SER | GLN | GLN | SER | SER | ALA | ALA | ||||
5 | GLN | GLN | SER | SER | VAL | ALA | GLN | SER | GLN | GLN | ||||
6 | THR | SER | TYR | SER | ALA | ALA | THR | ASN | ALA | GLY | ||||
7 | SER | SER | VAL | SER | GLN | SER | GLN | ALA | ILE | VAL | ||||
8 | SER | SER | ALA | PRO | VAL | TYR | PHE | ASN | SER | GLN | ||||
9 | THR | LEU | THR | ASN | ASN | LEU | ALA | SER | SER | LEU | ||||
10 | GLN | SER | LEU | ASN | ALA | LEU | ASN | TYR | VAL | SER | ||||
11 | ASN | GLY | GLN | LEU | SER | SER | SER | ASP | VAL | ALA | ||||
12 | SER | THR | VAL | ALA | ARG | ALA | VAL | ALA | GLN | SER | ||||
13 | LEU | GLY | LEU | SER | GLN | GLY | SER | VAL | GLN | ASN | ||||
14 | ILE | MET | SER | GLN | GLN | LEU | SER | SER | ILE | GLY | ||||
15 | SER | GLY | ALA | SER | THR | SER | SER | LEU | SER | GLN | ||||
16 | ALA | ILE | ALA | ASN | ALA | VAL | SER | SER | ALA | VAL | ||||
17 | GLN | GLY | SER | GLN | ALA | ALA | ALA | PRO | GLY | GLN | ||||
18 | GLU | GLN | SER | ILE | ALA | GLN | ARG | VAL | ASN | SER | ||||
19 | ALA | ILE | SER | SER | ALA | PHE | ALA | GLN | LEU | ILE | ||||
20 | SER | GLN | ARG | THR | ALA | PRO | ALA | PRO | ALA | PRO | ||||
21 | ARG | PRO | ARG | PRO | ALA | PRO | LEU | PRO | ALA | PRO | ||||
22 | ALA | PRO | ARG | PRO | ARG | PRO | ALA | PRO | ALA | PRO | ||||
23 | ARG | PRO | ALA | PRO | VAL | TYR | ALA | PRO | ALA | PRO | ||||
24 | VAL | ALA | SER | GLN | PHE | GLN | ALA | SER | ALA | SER | ||||
25 | SER | GLN | SER |
Samples:
sample_1: 1 mM DSS 1.0 mM; 1 mM NaN3 1.0 mM; HPy1_v2, [U-100% 13C; U-100% 15N], 0.5 mM
sample_conditions_1: ionic strength: 0.050 M; pH: 6.000; pressure: 1.000 atm; temperature: 313.150 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
HNcaCO (HNcaCO) | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
CBCAcoNH (H[N[co[{CA|ca[C]}]]]) | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
hcanco (hCANCO) | sample_1 | isotropic | sample_conditions_1 |
hcacon (h{CA|Cca}CO[N]) | sample_1 | isotropic | sample_conditions_1 |
hcaco (haCACO) | sample_1 | isotropic | sample_conditions_1 |
con v3 (CO[N]) | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr Analysis v2.4 - chemical shift assignment, data analysis
DANGLE v1.1 - data analysis
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts