BMRB Entry 51689
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51689
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Title: Assignments of human CAPRIN1 residues 607-709 N623TN630T mutant PubMed: 36018482
Deposition date: 2022-11-08 Original release date: 2022-11-10
Authors: Toyama, Yuki; Rangadurai, Atul Kaushik; Kay, Lewis
Citation: Toyama, Yuki; Rangadurai, Atul Kaushik; Kay, Lewis. "Measurement of 1Halpha transverse relaxation rates in proteins: application to solvent PREs" J. Biomol. NMR 76, 137-152 (2022).
Assembly members:
entity_1, polymer, 103 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: petSUMO
Entity Sequences (FASTA):
entity_1: SRGVSRGGSRGARGLMNGYR
GPANGFRGGYDGYRPSFSNT
PNSGYTQSQFSAPRDYSGYQ
RDGYQQNFKRGSGQSGPRGA
PRGRGGPPRPNRGMPQMNTQ
QVN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 91 |
| 15N chemical shifts | 91 |
| 1H chemical shifts | 91 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Caprin1 | 1 |
Entities:
Entity 1, Caprin1 103 residues - Formula weight is not available
| 1 | SER | ARG | GLY | VAL | SER | ARG | GLY | GLY | SER | ARG | ||||
| 2 | GLY | ALA | ARG | GLY | LEU | MET | THR | GLY | TYR | ARG | ||||
| 3 | GLY | PRO | ALA | THR | GLY | PHE | ARG | GLY | GLY | TYR | ||||
| 4 | ASP | GLY | TYR | ARG | PRO | SER | PHE | SER | ASN | THR | ||||
| 5 | PRO | ASN | SER | GLY | TYR | THR | GLN | SER | GLN | PHE | ||||
| 6 | SER | ALA | PRO | ARG | ASP | TYR | SER | GLY | TYR | GLN | ||||
| 7 | ARG | ASP | GLY | TYR | GLN | GLN | ASN | PHE | LYS | ARG | ||||
| 8 | GLY | SER | GLY | GLN | SER | GLY | PRO | ARG | GLY | ALA | ||||
| 9 | PRO | ARG | GLY | ARG | GLY | GLY | PRO | PRO | ARG | PRO | ||||
| 10 | ASN | ARG | GLY | MET | PRO | GLN | MET | ASN | THR | GLN | ||||
| 11 | GLN | VAL | ASN |
Samples:
sample_1: Caprin1 residues 607-709 N623TN630T, [U-100% 13C; U-100% 15N], 300 uM; MES 25 mM
sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNN | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRDraw v10.9 - processing
NMRFAM-SPARKY v1.470 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts