BMRB Entry 51697
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51697
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Title: Assignment of pre-miRNA-31 TopA short fragment PubMed: 36711709
Deposition date: 2022-11-18 Original release date: 2023-02-08
Authors: Kotar, Anita; Keane, Sarah
Citation: Ma, Sicong; Kotar, Anita; Grote, Scott; Rouskin, Silvi; Keane, Sarah. "Structure of pre-miR-31 reveals an active role in Dicer processing" bioRxiv ., .-. (2023).
Assembly members:
entity_1, polymer, 20 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: enzymatic semisynthesis
Entity Sequences (FASTA):
entity_1: GGCAUAGCUACGGCUAUGCC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 23 |
| 1H chemical shifts | 90 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pre-miRNA fragment | 1 |
Entities:
Entity 1, pre-miRNA fragment 20 residues - Formula weight is not available
| 1 | G | G | C | A | U | A | G | C | U | A | |
| 2 | C | G | G | C | U | A | U | G | C | C |
Samples:
sample_1: pre-miRNA fragment 0.4 mM; D2O, [U-100% 2H], 100%; potassium phosphate 50 mM; MgCl2 1 mM
sample_conditions_1: ionic strength: 0.0025 M; pH: 7.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRViewJ v9.2.0-b24 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz