BMRB Entry 51708
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51708
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Title: Solution state chemical shift NMR assignment of human l-III immunoglobulin light chain FOR005_GL PubMed: 37353525
Deposition date: 2022-11-24 Original release date: 2023-05-16
Authors: Pradhan, Tejaswini; Sarkar, Riddhiman; Zacharias, Martin; Reif, Bernd
Citation: Pradhan, Tejaswini; Sarkar, Riddhiman; Meighen-Berger, Kevin; Feige, Matthias; Zacharias, Martin; Reif, Bernd. "Mechanistic insights into the aggregation pathway of the patient-derived immunoglobulin light chain variable domain protein FOR005" Nat. commun. 14, 3755-3755 (2023).
Assembly members:
entity_1, polymer, 110 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 28(b+)
Entity Sequences (FASTA):
entity_1: GSSELTQDPAVSVALGQTVR
ITCQGDSLRSYYASWYQQKP
GQAPVLVIYGKNNRPSGIPD
RFSGSSSGNTASLTITGAQA
EDEADYYCNSRDSSGNHQVF
GGGTKLTVLG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 190 |
| 15N chemical shifts | 101 |
| 1H chemical shifts | 101 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VL | 1 |
Entities:
Entity 1, VL 110 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLU | LEU | THR | GLN | ASP | PRO | ALA | |
| 2 | VAL | SER | VAL | ALA | LEU | GLY | GLN | THR | VAL | ARG | |
| 3 | ILE | THR | CYS | GLN | GLY | ASP | SER | LEU | ARG | SER | |
| 4 | TYR | TYR | ALA | SER | TRP | TYR | GLN | GLN | LYS | PRO | |
| 5 | GLY | GLN | ALA | PRO | VAL | LEU | VAL | ILE | TYR | GLY | |
| 6 | LYS | ASN | ASN | ARG | PRO | SER | GLY | ILE | PRO | ASP | |
| 7 | ARG | PHE | SER | GLY | SER | SER | SER | GLY | ASN | THR | |
| 8 | ALA | SER | LEU | THR | ILE | THR | GLY | ALA | GLN | ALA | |
| 9 | GLU | ASP | GLU | ALA | ASP | TYR | TYR | CYS | ASN | SER | |
| 10 | ARG | ASP | SER | SER | GLY | ASN | HIS | GLN | VAL | PHE | |
| 11 | GLY | GLY | GLY | THR | LYS | LEU | THR | VAL | LEU | GLY |
Samples:
sample_1: Immunoglobulin light chain variable domain, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 50 mM; sodium phosphate 20 mM
sample_conditions_1: ionic strength: 0.07 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.1.1 - collection, processing
CcpNMR vv2 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts