BMRB Entry 51708

Name: Solution state chemical shift NMR assignment of human l-III immunoglobulin light chain FOR005_GL
Published: 2023-05-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51708

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution state chemical shift NMR assignment of human l-III immunoglobulin light chain FOR005_GL

Deposition date:

2022-11-24

Original release date:

2023-05-16

Authors:

Pradhan, Tejaswini; Sarkar, Riddhiman; Zacharias, Martin; Reif, Bernd

Citation:

Citation: Pradhan, Tejaswini; Sarkar, Riddhiman; Meighen-Berger, Kevin; Feige, Matthias; Zacharias, Martin; Reif, Bernd. "Mechanistic insights into the aggregation pathway of the patient-derived immunoglobulin light chain variable domain protein FOR005" Nat. commun. 14, 3755-3755 (2023).
PubMed: 37353525

Assembly members:

Assembly members:
entity_1, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 28(b+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSSELTQDPAVSVALGQTVR ITCQGDSLRSYYASWYQQKP GQAPVLVIYGKNNRPSGIPD RFSGSSSGNTASLTITGAQA EDEADYYCNSRDSSGNHQVF GGGTKLTVLG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	190
15N chemical shifts	101
1H chemical shifts	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	VL	1

Entities:

Entity 1, VL 110 residues - Formula weight is not available

1	GLY	SER	SER	GLU	LEU	THR	GLN	ASP	PRO	ALA
2	VAL	SER	VAL	ALA	LEU	GLY	GLN	THR	VAL	ARG
3	ILE	THR	CYS	GLN	GLY	ASP	SER	LEU	ARG	SER
4	TYR	TYR	ALA	SER	TRP	TYR	GLN	GLN	LYS	PRO
5	GLY	GLN	ALA	PRO	VAL	LEU	VAL	ILE	TYR	GLY
6	LYS	ASN	ASN	ARG	PRO	SER	GLY	ILE	PRO	ASP
7	ARG	PHE	SER	GLY	SER	SER	SER	GLY	ASN	THR
8	ALA	SER	LEU	THR	ILE	THR	GLY	ALA	GLN	ALA
9	GLU	ASP	GLU	ALA	ASP	TYR	TYR	CYS	ASN	SER
10	ARG	ASP	SER	SER	GLY	ASN	HIS	GLN	VAL	PHE
11	GLY	GLY	GLY	THR	LYS	LEU	THR	VAL	LEU	GLY

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 51708

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	GLY	SER	SER	GLU	LEU	THR	GLN	ASP	PRO	ALA
2	VAL	SER	VAL	ALA	LEU	GLY	GLN	THR	VAL	ARG
3	ILE	THR	CYS	GLN	GLY	ASP	SER	LEU	ARG	SER
4	TYR	TYR	ALA	SER	TRP	TYR	GLN	GLN	LYS	PRO
5	GLY	GLN	ALA	PRO	VAL	LEU	VAL	ILE	TYR	GLY
6	LYS	ASN	ASN	ARG	PRO	SER	GLY	ILE	PRO	ASP
7	ARG	PHE	SER	GLY	SER	SER	SER	GLY	ASN	THR
8	ALA	SER	LEU	THR	ILE	THR	GLY	ALA	GLN	ALA
9	GLU	ASP	GLU	ALA	ASP	TYR	TYR	CYS	ASN	SER
10	ARG	ASP	SER	SER	GLY	ASN	HIS	GLN	VAL	PHE
11	GLY	GLY	GLY	THR	LYS	LEU	THR	VAL	LEU	GLY

1	GLY	SER	SER	GLU	LEU	THR	GLN	ASP	PRO	ALA
2	VAL	SER	VAL	ALA	LEU	GLY	GLN	THR	VAL	ARG
3	ILE	THR	CYS	GLN	GLY	ASP	SER	LEU	ARG	SER
4	TYR	TYR	ALA	SER	TRP	TYR	GLN	GLN	LYS	PRO
5	GLY	GLN	ALA	PRO	VAL	LEU	VAL	ILE	TYR	GLY
6	LYS	ASN	ASN	ARG	PRO	SER	GLY	ILE	PRO	ASP
7	ARG	PHE	SER	GLY	SER	SER	SER	GLY	ASN	THR
8	ALA	SER	LEU	THR	ILE	THR	GLY	ALA	GLN	ALA
9	GLU	ASP	GLU	ALA	ASP	TYR	TYR	CYS	ASN	SER
10	ARG	ASP	SER	SER	GLY	ASN	HIS	GLN	VAL	PHE
11	GLY	GLY	GLY	THR	LYS	LEU	THR	VAL	LEU	GLY

1	GLY	SER	SER	GLU	LEU	THR	GLN	ASP	PRO	ALA
2	VAL	SER	VAL	ALA	LEU	GLY	GLN	THR	VAL	ARG
3	ILE	THR	CYS	GLN	GLY	ASP	SER	LEU	ARG	SER
4	TYR	TYR	ALA	SER	TRP	TYR	GLN	GLN	LYS	PRO
5	GLY	GLN	ALA	PRO	VAL	LEU	VAL	ILE	TYR	GLY
6	LYS	ASN	ASN	ARG	PRO	SER	GLY	ILE	PRO	ASP
7	ARG	PHE	SER	GLY	SER	SER	SER	GLY	ASN	THR
8	ALA	SER	LEU	THR	ILE	THR	GLY	ALA	GLN	ALA
9	GLU	ASP	GLU	ALA	ASP	TYR	TYR	CYS	ASN	SER
10	ARG	ASP	SER	SER	GLY	ASN	HIS	GLN	VAL	PHE
11	GLY	GLY	GLY	THR	LYS	LEU	THR	VAL	LEU	GLY