BMRB Entry 51710
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51710
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: SARS-CoV-2 Phosphorylated Nucleocapsid 1-209 at 35C PubMed: 36807437
Deposition date: 2022-11-26 Original release date: 2023-03-07
Authors: Lee, Eunjeong
Citation: Lee, Eunjeong; Redzic, Jasmina; Saviola, Anthony; Li, Xueni; Ebmeier, Christopher; Kutateladze, Tatiana; Hansen, Kirk Charles; Zhao, Rui; Ahn, Natalie; Sluchanko, Nikolai; Eisenmesser, Elan. "Molecular insight into the specific interactions of the SARS-CoV-2 Nucleocapsid with RNA and host protein" Protein Sci 32, e4603-e4603 (2023).
Assembly members:
entity_1, polymer, 209 residues, Formula weight is not available
Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29
Entity Sequences (FASTA):
entity_1: MSDNGPQNQRNAPRITFGGP
SDSTGSNQNGERSGARSKQR
RPQGLPNNTASWFTALTQHG
KEDLKFPRGQGVPINTNSSP
DDQIGYYRRATRRIRGGDGK
MKDLSPRWYFYYLGTGPEAG
LPYGANKDGIIWVATEGALN
TPKDHIGTRNPANNAAIVLQ
LPQGTTLPKGFYAEGSRGGS
QASSRSSSRSRNSSRNSTPG
SSRGTSPAR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 185 |
| 15N chemical shifts | 166 |
| 1H chemical shifts | 166 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SARS-Cov-2 Phosphorylated Nucleocapsid protein | 1 |
Entities:
Entity 1, SARS-Cov-2 Phosphorylated Nucleocapsid protein 209 residues - Formula weight is not available
| 1 | MET | SER | ASP | ASN | GLY | PRO | GLN | ASN | GLN | ARG | ||||
| 2 | ASN | ALA | PRO | ARG | ILE | THR | PHE | GLY | GLY | PRO | ||||
| 3 | SER | ASP | SER | THR | GLY | SER | ASN | GLN | ASN | GLY | ||||
| 4 | GLU | ARG | SER | GLY | ALA | ARG | SER | LYS | GLN | ARG | ||||
| 5 | ARG | PRO | GLN | GLY | LEU | PRO | ASN | ASN | THR | ALA | ||||
| 6 | SER | TRP | PHE | THR | ALA | LEU | THR | GLN | HIS | GLY | ||||
| 7 | LYS | GLU | ASP | LEU | LYS | PHE | PRO | ARG | GLY | GLN | ||||
| 8 | GLY | VAL | PRO | ILE | ASN | THR | ASN | SER | SER | PRO | ||||
| 9 | ASP | ASP | GLN | ILE | GLY | TYR | TYR | ARG | ARG | ALA | ||||
| 10 | THR | ARG | ARG | ILE | ARG | GLY | GLY | ASP | GLY | LYS | ||||
| 11 | MET | LYS | ASP | LEU | SER | PRO | ARG | TRP | TYR | PHE | ||||
| 12 | TYR | TYR | LEU | GLY | THR | GLY | PRO | GLU | ALA | GLY | ||||
| 13 | LEU | PRO | TYR | GLY | ALA | ASN | LYS | ASP | GLY | ILE | ||||
| 14 | ILE | TRP | VAL | ALA | THR | GLU | GLY | ALA | LEU | ASN | ||||
| 15 | THR | PRO | LYS | ASP | HIS | ILE | GLY | THR | ARG | ASN | ||||
| 16 | PRO | ALA | ASN | ASN | ALA | ALA | ILE | VAL | LEU | GLN | ||||
| 17 | LEU | PRO | GLN | GLY | THR | THR | LEU | PRO | LYS | GLY | ||||
| 18 | PHE | TYR | ALA | GLU | GLY | SER | ARG | GLY | GLY | SER | ||||
| 19 | GLN | ALA | SER | SER | ARG | SER | SER | SER | ARG | SER | ||||
| 20 | ARG | ASN | SER | SER | ARG | ASN | SER | THR | PRO | GLY | ||||
| 21 | SER | SER | ARG | GLY | THR | SER | PRO | ALA | ARG |
Samples:
sample_1: Sars-Cov-2 Nucleocapsid, [U-100% 15N], 500 uM; sodium chloride 150 mM; HEPES 50 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts