BMRB Entry 51723
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PDB ID: 8hn2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51723
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Kishimoto, Hiraku; Azai, Chihiro; Yamamoto, Tomoya; Mutoh, Risa; Nakaniwa, Tetsuko; Tanaka, Hideaki; Miyanoiri, Yohei; Kurisu, Genji; Oh-oka, Hirozo. "Soluble domains of cytochrome c-556 and Rieske iron-sulfur protein from Chlorobaculum tepidu m: Crystal structures and interaction analysis" Curr. Res. Struct. Biol. 5, 100101-100101 (2023).
PubMed: 37180033
Assembly members:
entity_1, polymer, 119 residues, 12798 Da.
entity_FES, non-polymer, 175.820 Da.
Natural source: Common Name: green sulfur bacteria Taxonomy ID: 1097 Superkingdom: Bacteria Kingdom: not available Genus/species: Chlorobaculum tepidum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a
Entity Sequences (FASTA):
entity_1: AKKIKIVNELAVGPASDVPN
GTGKIYQFNDDKVIVVNHGG
SLTAVSAICTHLGCLVHWDE
AADMIACPCHGAKYRQDGKI
ISGPQPLPLKQYKVKIEDGK
IVVSIAKLAAALEHHHHHH
| Data type | Count |
| 13C chemical shifts | 1338 |
| 15N chemical shifts | 609 |
| 1H chemical shifts | 609 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rieske protein | 1 |
| 2 | [2Fe-2S] | 2 |
Entities:
Entity 1, Rieske protein 119 residues - 12798 Da.
Residues 182-194 is Histidine tag. This is the soluble domain (Residues 76-181) of a transmembrane protein.
| 1 | ALA | LYS | LYS | ILE | LYS | ILE | VAL | ASN | GLU | LEU | ||||
| 2 | ALA | VAL | GLY | PRO | ALA | SER | ASP | VAL | PRO | ASN | ||||
| 3 | GLY | THR | GLY | LYS | ILE | TYR | GLN | PHE | ASN | ASP | ||||
| 4 | ASP | LYS | VAL | ILE | VAL | VAL | ASN | HIS | GLY | GLY | ||||
| 5 | SER | LEU | THR | ALA | VAL | SER | ALA | ILE | CYS | THR | ||||
| 6 | HIS | LEU | GLY | CYS | LEU | VAL | HIS | TRP | ASP | GLU | ||||
| 7 | ALA | ALA | ASP | MET | ILE | ALA | CYS | PRO | CYS | HIS | ||||
| 8 | GLY | ALA | LYS | TYR | ARG | GLN | ASP | GLY | LYS | ILE | ||||
| 9 | ILE | SER | GLY | PRO | GLN | PRO | LEU | PRO | LEU | LYS | ||||
| 10 | GLN | TYR | LYS | VAL | LYS | ILE | GLU | ASP | GLY | LYS | ||||
| 11 | ILE | VAL | VAL | SER | ILE | ALA | LYS | LEU | ALA | ALA | ||||
| 12 | ALA | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Entity 2, [2Fe-2S] - Fe2 S2 - 175.820 Da.
| 1 | FES |
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