BMRB Entry 51741
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51741
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Title: EWS RRM-RG2-ZnF PubMed: 36929870
Deposition date: 2022-12-15 Original release date: 2023-05-24
Authors: Selig, Emily; Xu, Xiaoping; Libich, David
Citation: Selig, Emily; Bhura, Roohi; White, Matthew; Akula, Shivani; Hoffman, Renee; Tovar, Carmel; Xu, Xiaoping; Booth, Rachell; Libich, David. "Biochemical and biophysical characterization of the nucleic acid binding properties of the RNA/DNA binding protein EWS" Biopolymers 114, e23536-e23536 (2023).
Assembly members:
entity_1, polymer, 193 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
Entity Sequences (FASTA):
entity_1: GADNSAIYVQGLNDSVTLDD
LADFFKQCGVVKMNKRTGQP
MIHIYLDKETGKPKGDATVS
YEDPPTAKAAVEWFDGKDFQ
GSKLKVSLARKKPPMNSMRG
GLPPREGRGMPPPLRGGPGG
PGGPGGPMGRMGGRGGDRGG
FPPRGPRGSRGNPSGGGNVQ
HRAGDWQCPNPGCGNQNFAW
RTECNQCKAPKPE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 382 |
| 15N chemical shifts | 132 |
| 1H chemical shifts | 132 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RRM-RG2-ZnF | 1 |
| 2 | zinc ion | 2 |
Entities:
Entity 1, RRM-RG2-ZnF 193 residues - Formula weight is not available
| 1 | GLY | ALA | ASP | ASN | SER | ALA | ILE | TYR | VAL | GLN | ||||
| 2 | GLY | LEU | ASN | ASP | SER | VAL | THR | LEU | ASP | ASP | ||||
| 3 | LEU | ALA | ASP | PHE | PHE | LYS | GLN | CYS | GLY | VAL | ||||
| 4 | VAL | LYS | MET | ASN | LYS | ARG | THR | GLY | GLN | PRO | ||||
| 5 | MET | ILE | HIS | ILE | TYR | LEU | ASP | LYS | GLU | THR | ||||
| 6 | GLY | LYS | PRO | LYS | GLY | ASP | ALA | THR | VAL | SER | ||||
| 7 | TYR | GLU | ASP | PRO | PRO | THR | ALA | LYS | ALA | ALA | ||||
| 8 | VAL | GLU | TRP | PHE | ASP | GLY | LYS | ASP | PHE | GLN | ||||
| 9 | GLY | SER | LYS | LEU | LYS | VAL | SER | LEU | ALA | ARG | ||||
| 10 | LYS | LYS | PRO | PRO | MET | ASN | SER | MET | ARG | GLY | ||||
| 11 | GLY | LEU | PRO | PRO | ARG | GLU | GLY | ARG | GLY | MET | ||||
| 12 | PRO | PRO | PRO | LEU | ARG | GLY | GLY | PRO | GLY | GLY | ||||
| 13 | PRO | GLY | GLY | PRO | GLY | GLY | PRO | MET | GLY | ARG | ||||
| 14 | MET | GLY | GLY | ARG | GLY | GLY | ASP | ARG | GLY | GLY | ||||
| 15 | PHE | PRO | PRO | ARG | GLY | PRO | ARG | GLY | SER | ARG | ||||
| 16 | GLY | ASN | PRO | SER | GLY | GLY | GLY | ASN | VAL | GLN | ||||
| 17 | HIS | ARG | ALA | GLY | ASP | TRP | GLN | CYS | PRO | ASN | ||||
| 18 | PRO | GLY | CYS | GLY | ASN | GLN | ASN | PHE | ALA | TRP | ||||
| 19 | ARG | THR | GLU | CYS | ASN | GLN | CYS | LYS | ALA | PRO | ||||
| 20 | LYS | PRO | GLU |
Entity 2, zinc ion - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: EWS RRM-RG2-ZnF monomer, [U-99% 15N], 400 uM; potassium phosphate 20 mM; potassium chloride 150 mM; zinc chloride 0.5 mM; TCEP 2 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCANH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v3.1 - chemical shift assignment
NMRPipe - processing
SMILE - processing
NMRDraw - processing
TOPSPIN v4.1.1 - collection, processing
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts