BMRB Entry 51786
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51786
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Rec114:Mei4 minimal structured complex at pH 6.1 PubMed: 36711595
Deposition date: 2023-01-18 Original release date: 2023-03-07
Authors: Liu, Kaixian; Grasso, Emily; Pu, Stephen; Liu, Shixin; Eliezer, David; Keeney, Scott
Citation: Liu, Kaixian; Grasso, Emily; Pu, Stephen; Liu, Shixin; Eliezer, David; Keeney, Scott. "Structure and DNA bridging activity of the essential Rec114-Mei4 trimer interface" bioRxiv ., .-. (2023).
Assembly members:
entity_1, polymer, 32 residues, Formula weight is not available
entity_2, polymer, 42 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet
Entity Sequences (FASTA):
entity_1: SETSEVDWIICFALIQSRNP
TLWKRALSRKKG
entity_2: MNVNRNASRKISKRLIKEKL
KDEEFIKWVNKVETVLNKMF
EK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 338 |
| 15N chemical shifts | 109 |
| 1H chemical shifts | 109 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mei4 | 1 |
| 2 | Rec114 | 2 |
| 3 | Rec114b | 2 |
Entities:
Entity 1, Mei4 32 residues - Formula weight is not available
| 1 | SER | GLU | THR | SER | GLU | VAL | ASP | TRP | ILE | ILE | ||||
| 2 | CYS | PHE | ALA | LEU | ILE | GLN | SER | ARG | ASN | PRO | ||||
| 3 | THR | LEU | TRP | LYS | ARG | ALA | LEU | SER | ARG | LYS | ||||
| 4 | LYS | GLY |
Entity 2, Rec114 42 residues - Formula weight is not available
| 1 | MET | ASN | VAL | ASN | ARG | ASN | ALA | SER | ARG | LYS | ||||
| 2 | ILE | SER | LYS | ARG | LEU | ILE | LYS | GLU | LYS | LEU | ||||
| 3 | LYS | ASP | GLU | GLU | PHE | ILE | LYS | TRP | VAL | ASN | ||||
| 4 | LYS | VAL | GLU | THR | VAL | LEU | ASN | LYS | MET | PHE | ||||
| 5 | GLU | LYS |
Samples:
sample_1: Mei4, [U-100% 13C; U-100% 15N], 470 uM; Rec114, [U-100% 13C; U-100% 15N], 940 uM; sodium phosphate 25 mM; sodium chloride 100 mM; D2O 5.5%%; EDTA 0.5 mM; sodium azide .05%
sample_conditions_1: pH: 6.1; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNN | sample_1 | isotropic | sample_conditions_1 |
Software:
TALOS-N - data analysis
NMRbox - data analysis, processing
NMRFAM-SPARKY - data analysis
NMRPipe - processing
TOPSPIN v3 - collection
SMILE - processing
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts