BMRB Entry 51786

Name: Rec114:Mei4 minimal structured complex at pH 6.1
Published: 2023-03-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51786

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Rec114:Mei4 minimal structured complex at pH 6.1

Deposition date:

2023-01-18

Original release date:

2023-03-07

Authors:

Liu, Kaixian; Grasso, Emily; Pu, Stephen; Liu, Shixin; Eliezer, David; Keeney, Scott

Citation:

Citation: Liu, Kaixian; Grasso, Emily; Pu, Stephen; Liu, Shixin; Eliezer, David; Keeney, Scott. "Structure and DNA bridging activity of the essential Rec114-Mei4 trimer interface" bioRxiv ., .-. (2023).
PubMed: 36711595

Assembly members:

Assembly members:
entity_1, polymer, 32 residues, Formula weight is not available
entity_2, polymer, 42 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SETSEVDWIICFALIQSRNP TLWKRALSRKKG
entity_2: MNVNRNASRKISKRLIKEKL KDEEFIKWVNKVETVLNKMF EK

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	338
15N chemical shifts	109
1H chemical shifts	109

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Mei4	1
2	Rec114	2
3	Rec114b	2

Entities:

Entity 1, Mei4 32 residues - Formula weight is not available

1

SER

GLU

THR

SER

GLU

VAL

ASP

TRP

ILE

2

CYS

PHE

ALA

LEU

ILE

GLN

SER

ARG

ASN

PRO

3

THR

LEU

TRP

LYS

ARG

ALA

LEU

SER

ARG

LYS

4

LYS

GLY

Entity 2, Rec114 42 residues - Formula weight is not available

1

MET

ASN

VAL

ASN

ARG

ASN

ALA

SER

ARG

LYS

2

ILE

SER

LYS

ARG

LEU

ILE

LYS

GLU

LYS

LEU

3

LYS

ASP

GLU

PHE

ILE

LYS

TRP

VAL

ASN

4

LYS

VAL

GLU

THR

VAL

LEU

ASN

LYS

MET

PHE

5

GLU

LYS

Samples:

sample_1: Mei4, [U-100% 13C; U-100% 15N], 470 uM; Rec114, [U-100% 13C; U-100% 15N], 940 uM; sodium phosphate 25 mM; sodium chloride 100 mM; D2O 5.5%%; EDTA 0.5 mM; sodium azide .05%

sample_conditions_1: pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNN	sample_1	isotropic	sample_conditions_1

Software:

TALOS-N - data analysis

NMRbox - data analysis, processing

NMRFAM-SPARKY - data analysis

NMRPipe - processing

TOPSPIN v3 - collection

SMILE - processing

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks