BMRB Entry 51787

Title:
Rec114:Mei4 minimal structured complex at pH 7.4
Deposition date:
2023-01-18
Original release date:
2023-03-07
Authors:
Liu, Kaixian; Grasso, Emily; Pu, Stephen; Liu, Shixin; Eliezer, David; Keeney, Scott
Citation:

Citation: Liu, Kaixian; Grasso, Emily; Pu, Stephen; Liu, Shixin; Eliezer, David; Keeney, Scott. "Structure and DNA bridging activity of the essential Rec114-Mei4 trimer interface"  bioRxiv ., .-. (2023).
PubMed: 36711595

Assembly members:

Assembly members:
entity_1, polymer, 43 residues, Formula weight is not available
entity_2, polymer, 55 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts291
15N chemical shifts93
1H chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Mei41
2Rec1142
3Rec114b2

Entities:

Entity 1, Mei4 43 residues - Formula weight is not available

1   METSERARGGLYLYSLEUGLUASPMETGLU
2   GLNLYSGLUTHRSERGLUVALASPTRPILE
3   ILECYSPHEALALEUILEGLNSERARGASN
4   PROTHRLEUTRPLYSARGALALEUSERARG
5   LYSLYSGLY

Entity 2, Rec114 55 residues - Formula weight is not available

1   SERGLUALASERPROSERGLUGLYLEUTHR
2   ILEARGVALLYSASNVALASNARGASNALA
3   SERARGLYSILESERLYSARGLEUILELYS
4   GLULYSLEULYSASPGLUGLUPHEILELYS
5   TRPVALASNLYSVALGLUTHRVALLEUASN
6   LYSMETPHEGLULYS

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