BMRB Entry 51787
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51787
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Title: Rec114:Mei4 minimal structured complex at pH 7.4 PubMed: 36711595
Deposition date: 2023-01-18 Original release date: 2023-03-07
Authors: Liu, Kaixian; Grasso, Emily; Pu, Stephen; Liu, Shixin; Eliezer, David; Keeney, Scott
Citation: Liu, Kaixian; Grasso, Emily; Pu, Stephen; Liu, Shixin; Eliezer, David; Keeney, Scott. "Structure and DNA bridging activity of the essential Rec114-Mei4 trimer interface" bioRxiv ., .-. (2023).
Assembly members:
entity_1, polymer, 43 residues, Formula weight is not available
entity_2, polymer, 55 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet
Entity Sequences (FASTA):
entity_1: MSRGKLEDMEQKETSEVDWI
ICFALIQSRNPTLWKRALSR
KKG
entity_2: SEASPSEGLTIRVKNVNRNA
SRKISKRLIKEKLKDEEFIK
WVNKVETVLNKMFEK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 291 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 93 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Mei4 | 1 |
| 2 | Rec114 | 2 |
| 3 | Rec114b | 2 |
Entities:
Entity 1, Mei4 43 residues - Formula weight is not available
| 1 | MET | SER | ARG | GLY | LYS | LEU | GLU | ASP | MET | GLU | ||||
| 2 | GLN | LYS | GLU | THR | SER | GLU | VAL | ASP | TRP | ILE | ||||
| 3 | ILE | CYS | PHE | ALA | LEU | ILE | GLN | SER | ARG | ASN | ||||
| 4 | PRO | THR | LEU | TRP | LYS | ARG | ALA | LEU | SER | ARG | ||||
| 5 | LYS | LYS | GLY |
Entity 2, Rec114 55 residues - Formula weight is not available
| 1 | SER | GLU | ALA | SER | PRO | SER | GLU | GLY | LEU | THR | ||||
| 2 | ILE | ARG | VAL | LYS | ASN | VAL | ASN | ARG | ASN | ALA | ||||
| 3 | SER | ARG | LYS | ILE | SER | LYS | ARG | LEU | ILE | LYS | ||||
| 4 | GLU | LYS | LEU | LYS | ASP | GLU | GLU | PHE | ILE | LYS | ||||
| 5 | TRP | VAL | ASN | LYS | VAL | GLU | THR | VAL | LEU | ASN | ||||
| 6 | LYS | MET | PHE | GLU | LYS |
Samples:
sample_1: Mei4, [U-100% 13C; U-100% 15N], 600 uM; Rec114, [U-100% 13C; U-100% 15N], 1200 uM; HEPES 25 mM; sodium chloride 100 mM; D2O 5%%; EDTA 0.5 mM; sodium azide .05%; TCEP 1 mM
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNN | sample_1 | isotropic | sample_conditions_1 |
| 3D HCC(CO)NH TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TALOS-N - data analysis
NMRbox - data analysis, processing
NMRFAM-SPARKY - data analysis
NMRPipe - processing
TOPSPIN v3 - collection
SMILE - processing
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts