BMRB Entry 51805

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51805

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Title:
1H and 15N chemical shifts of WT RhoA bound to GMPPNP
Deposition date:
2023-01-25
Original release date:
2024-06-24
Authors:
Lin, Yuan; Ramelot, Theresa; Zheng, Yi
Citation:

Citation: Lin, Yuan; Ramelot, Theresa; Senyuz, Simge; Gursoy, Attila; Jang, Hyunbum; Nussinov, Ruth; Keskin, Ozlem; Zheng, Yi. "Tumor-derived RHOA mutants interact with effectors in the GDP-bound state"  Nat. Commun. 15, 7176-7176 (2024).
PubMed: 39169042

Assembly members:

Assembly members:
entity_1, polymer, 181 residues, Formula weight is not available
entity_GNP, non-polymer, 522.196 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAAIRKKLVIVGDGACGKTC LLIVNSKDQFPEVYVPTVFE NYVADIEVDGKQVELALWDT AGQEDYDRLRPLSYPDTDVI LMCFSIDSPDSLENIPEKWT PEVKHFCPNVPIILVGNKKD LRNDEHTRRELAKMKQEPVK PEEGRDMANRIGAFGYMECS AKTKDGVREVFEMATRAALQ A

Data sets:
Data typeCount
15N chemical shifts107
1H chemical shifts107

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RhoA1
2GMPPNP2

Entities:

Entity 1, RhoA 181 residues - Formula weight is not available

RhoA 1-181 (full length 1-193, truncated at 182), contains F25N mutation for better protein expression. non-native GS at N-terminus.

1   METALAALAILEARGLYSLYSLEUVALILE
2   VALGLYASPGLYALACYSGLYLYSTHRCYS
3   LEULEUILEVALASNSERLYSASPGLNPHE
4   PROGLUVALTYRVALPROTHRVALPHEGLU
5   ASNTYRVALALAASPILEGLUVALASPGLY
6   LYSGLNVALGLULEUALALEUTRPASPTHR
7   ALAGLYGLNGLUASPTYRASPARGLEUARG
8   PROLEUSERTYRPROASPTHRASPVALILE
9   LEUMETCYSPHESERILEASPSERPROASP
10   SERLEUGLUASNILEPROGLULYSTRPTHR
11   PROGLUVALLYSHISPHECYSPROASNVAL
12   PROILEILELEUVALGLYASNLYSLYSASP
13   LEUARGASNASPGLUHISTHRARGARGGLU
14   LEUALALYSMETLYSGLNGLUPROVALLYS
15   PROGLUGLUGLYARGASPMETALAASNARG
16   ILEGLYALAPHEGLYTYRMETGLUCYSSER
17   ALALYSTHRLYSASPGLYVALARGGLUVAL
18   PHEGLUMETALATHRARGALAALALEUGLN
19   ALA

Entity 2, GMPPNP - C10 H17 N6 O13 P3 - 522.196 Da.

1   GNP

Related Database Links:

UNP P61586

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks