BMRB Entry 51809

Name: Stem 1 hairpin with linker of Tytrahymena telomerase RNA
Published: 2023-06-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51809

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Stem 1 hairpin with linker of Tytrahymena telomerase RNA PubMed: 37330293

Deposition date: 2023-01-25 Original release date: 2023-06-19

Authors: Wang, Yaqiang; Feigon, Juli

Citation: Wang, Yaqiang; He, Yao; Wang, Yanjiao; Yang, Yuan; Singh, Mahavir; Eichhom, Catherine; Cheng, Xinyi; Jiang, Yi Xiao; Zhou, Z.Hong; Feigon, Juli. "Structure of LARP7 Protein p65-telomerase RNA Complex in Telomerase Revealed by Cryo-EM and NMR" J. Mol. Biol. 435, 168044-168044 (2023).

Assembly members:
entity_1, polymer, 21 residues, Formula weight is not available

Natural source: Common Name: Tetrahymena Thermophilia Taxonomy ID: 5911 Superkingdom: Eukaryota Kingdom: not available Genus/species: Tetrahymena Thermophilia

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
entity_1: AUACCCGCUUCGGCGGGACA A

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_2

Data type	Count
1H chemical shifts	9

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	stem 1 hairpin with linker of Tetrahymena telomerase RNA	1

Entities:

Entity 1, stem 1 hairpin with linker of Tetrahymena telomerase RNA 21 residues - Formula weight is not available

1

A

U

A

C

G

C

U

2

C

G

C

G

A

C

A

3

A

Samples:

sample_1: stem 1 hairpin of Tetrahymena telomerase RNA with ACAA linker 0.05 mM; D2O, [U-100% 2H], 10%; sodium phosphate 10 mM; potassium chloride 50 mM; H2O 90%

sample_conditions_1: ionic strength: 0.06 M; pH: 6.4; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRFAM-SPARKY - chemical shift assignment

NMRDraw - processing

NMRPipe - processing

NMR spectrometers:

Bruker AVANCE III 800 MHz