BMRB Entry 51812

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51812

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Title:
1H, 13C, 15N backbone chemical shift assignments of the extended ARID domain from human AT-rich interactive domain protein 5a (Arid5a)
Deposition date:
2023-01-30
Original release date:
2023-02-03
Authors:
von Ehr, Julian; Korn, Sophie; Schlundt, Andreas
Citation:

Citation: von Ehr, Julian; Korn, Sophie Marianne; Weiss, Lena; Schlundt, Andreas. "1H, 13C, 15N backbone chemical shift assignments of the extended ARID domain in human AT-rich interactive domain protein 5a (Arid5a)"  Biomol. NMR Assign. 17, 121-127 (2023).
PubMed: 37129704

Assembly members:

Assembly members:
entity_1, polymer, 151 residues, 17380 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-Trx1-a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMAISLEDSPEAGGEREEE QEREEEQAFLVSLYKFMKER HTPIERVPHLGFKQINLWKI YKAVEKLGAYELVTGRRLWK NVYDELGGSPGSTSAATCTR RHYERLVLPYVRHLKGEDDK PLPTSKPRKQYKMAKENRGD DGATERPKKAK

Data typeCount
13C chemical shifts283
15N chemical shifts288
1H chemical shifts300

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Arid5a 37-1831

Entities:

Entity 1, Arid5a 37-183 151 residues - 17380 Da.

The first four residues (GAMA) in the protein construct used are cloning artifacts.

1   GLYALAMETALAILESERLEUGLUASPSER
2   PROGLUALAGLYGLYGLUARGGLUGLUGLU
3   GLNGLUARGGLUGLUGLUGLNALAPHELEU
4   VALSERLEUTYRLYSPHEMETLYSGLUARG
5   HISTHRPROILEGLUARGVALPROHISLEU
6   GLYPHELYSGLNILEASNLEUTRPLYSILE
7   TYRLYSALAVALGLULYSLEUGLYALATYR
8   GLULEUVALTHRGLYARGARGLEUTRPLYS
9   ASNVALTYRASPGLULEUGLYGLYSERPRO
10   GLYSERTHRSERALAALATHRCYSTHRARG
11   ARGHISTYRGLUARGLEUVALLEUPROTYR
12   VALARGHISLEULYSGLYGLUASPASPLYS
13   PROLEUPROTHRSERLYSPROARGLYSGLN
14   TYRLYSMETALALYSGLUASNARGGLYASP
15   ASPGLYALATHRGLUARGPROLYSLYSALA
16   LYS

Related Database Links:

UNP Q03989-1
AlphaFold Q03989-1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks