BMRB Entry 51819

Name: The RRM-mediated RNA binding activity in T. brucei RAP1 is essential for VSG monoallelic expression
Published: 2023-02-16

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51819

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The RRM-mediated RNA binding activity in T. brucei RAP1 is essential for VSG monoallelic expression

Deposition date:

2023-02-03

Original release date:

2023-02-16

Authors:

Zhao, Yanxiang; Yang, Xian

Citation:

Citation: Gaurav, Amit Kumar; Afrin, Marjia; Yang, Xian; Saha, Arpita; Sayeed, S; Pan, Xuehua; Ji, Zeyang; Wong, Kam-Bo; Zhang, Mingjie; Zhao, Yanxiang; Li, Bibo. "The RRM-mediated RNA binding activity in T. brucei RAP1 is essential for VSG monoallelic expression" Nat. Commun. 14, 1576-1576 (2023).
PubMed: 36949076

Assembly members:

Assembly members:
entity_1, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPGSEATEEIAALDQPFEKC FIPTEALGSDREGLDRTQLE RQLPFRNYPIKLNVSKSGIF CQFPTVSDAKRFYEEGTVEI LNRSLPIKPVFEKRNETVAP AERKRRRSVSPGGVHPQTAA VSALSRR

Data sets:

spectral_peak_list

Data type

Count

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TbRAP1	1

Entities:

Entity 1, TbRAP1 127 residues - Formula weight is not available

1

GLY

PRO

GLY

SER

GLU

ALA

THR

GLU

ILE

2

ALA

LEU

ASP

GLN

PRO

PHE

GLU

LYS

CYS

3

PHE

ILE

PRO

THR

GLU

ALA

LEU

GLY

SER

ASP

4

ARG

GLU

GLY

LEU

ASP

ARG

THR

GLN

LEU

GLU

5

ARG

GLN

LEU

PRO

PHE

ARG

ASN

TYR

PRO

ILE

6

LYS

LEU

ASN

VAL

SER

LYS

SER

GLY

ILE

PHE

7

CYS

GLN

PHE

PRO

THR

VAL

SER

ASP

ALA

LYS

8

ARG

PHE

TYR

GLU

GLY

THR

VAL

GLU

ILE

9

LEU

ASN

ARG

SER

LEU

PRO

ILE

LYS

PRO

VAL

10

PHE

GLU

LYS

ARG

ASN

GLU

THR

VAL

ALA

PRO

11

ALA

GLU

ARG

LYS

ARG

SER

VAL

SER

12

PRO

GLY

VAL

HIS

PRO

GLN

THR

ALA

13

VAL

SER

ALA

LEU

SER

ARG

Samples:

sample_1: TbRAP1, [U-99% 15N], 0.1 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift calculation

NMR spectrometers:

Varian Avance 800 MHz