BMRB Entry 5182
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5182
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Extracellular Ligand-Binding Domain of Ionotropic Glutamate Receptor 2 PubMed: 11991359
Deposition date: 2001-10-17 Original release date: 2002-10-17
Authors: McFeeters, Robert; Swapna, G.V.T.; Montelione, Gaetano; Oswald, Robert
Citation: McFeeters, Robert; Swapna, G.V.T.; Montelione, Gaetano; Oswald, Robert. "Letter to the Editor: Semi-automated Backbone Resonance Assignments of the Extracellular Ligand-binding Domain of an Ionotropic Glutamate Receptor" J. Biomol. NMR 22, 297-298 (2002).
Assembly members:
ionotropic glutamate receptor 2, polymer, 263 residues, 29184 Da.
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
ionotropic glutamate receptor 2: GANKTVVVTTILESPYVMMK
KNHEMLEGNERYEGYCVDLA
AEIAKHCGFKYKLTIVGDGK
YGARDADTKIWNGMVGELVY
GKADIAIAPLTITLVREEVI
DFSKPFMSLGISIMIKKGTP
IESAEDLSKQTEIAYGTLDS
GSTKEFFRRSKIAVFDKMWT
YMRSAEPSVFVRTTAEGVAR
VRKSKGKYAYLLESTMNEYI
EQRKPCDTMKVGGNLDSKGY
GIATPKGSSLGNAVNLAVLK
LNEQGLLDKLKNKWWYDKGE
CGS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 740 |
| 15N chemical shifts | 246 |
| 1H chemical shifts | 246 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | gluR2 s1s2 | 1 |
Entities:
Entity 1, gluR2 s1s2 263 residues - 29184 Da.
| 1 | GLY | ALA | ASN | LYS | THR | VAL | VAL | VAL | THR | THR | ||||
| 2 | ILE | LEU | GLU | SER | PRO | TYR | VAL | MET | MET | LYS | ||||
| 3 | LYS | ASN | HIS | GLU | MET | LEU | GLU | GLY | ASN | GLU | ||||
| 4 | ARG | TYR | GLU | GLY | TYR | CYS | VAL | ASP | LEU | ALA | ||||
| 5 | ALA | GLU | ILE | ALA | LYS | HIS | CYS | GLY | PHE | LYS | ||||
| 6 | TYR | LYS | LEU | THR | ILE | VAL | GLY | ASP | GLY | LYS | ||||
| 7 | TYR | GLY | ALA | ARG | ASP | ALA | ASP | THR | LYS | ILE | ||||
| 8 | TRP | ASN | GLY | MET | VAL | GLY | GLU | LEU | VAL | TYR | ||||
| 9 | GLY | LYS | ALA | ASP | ILE | ALA | ILE | ALA | PRO | LEU | ||||
| 10 | THR | ILE | THR | LEU | VAL | ARG | GLU | GLU | VAL | ILE | ||||
| 11 | ASP | PHE | SER | LYS | PRO | PHE | MET | SER | LEU | GLY | ||||
| 12 | ILE | SER | ILE | MET | ILE | LYS | LYS | GLY | THR | PRO | ||||
| 13 | ILE | GLU | SER | ALA | GLU | ASP | LEU | SER | LYS | GLN | ||||
| 14 | THR | GLU | ILE | ALA | TYR | GLY | THR | LEU | ASP | SER | ||||
| 15 | GLY | SER | THR | LYS | GLU | PHE | PHE | ARG | ARG | SER | ||||
| 16 | LYS | ILE | ALA | VAL | PHE | ASP | LYS | MET | TRP | THR | ||||
| 17 | TYR | MET | ARG | SER | ALA | GLU | PRO | SER | VAL | PHE | ||||
| 18 | VAL | ARG | THR | THR | ALA | GLU | GLY | VAL | ALA | ARG | ||||
| 19 | VAL | ARG | LYS | SER | LYS | GLY | LYS | TYR | ALA | TYR | ||||
| 20 | LEU | LEU | GLU | SER | THR | MET | ASN | GLU | TYR | ILE | ||||
| 21 | GLU | GLN | ARG | LYS | PRO | CYS | ASP | THR | MET | LYS | ||||
| 22 | VAL | GLY | GLY | ASN | LEU | ASP | SER | LYS | GLY | TYR | ||||
| 23 | GLY | ILE | ALA | THR | PRO | LYS | GLY | SER | SER | LEU | ||||
| 24 | GLY | ASN | ALA | VAL | ASN | LEU | ALA | VAL | LEU | LYS | ||||
| 25 | LEU | ASN | GLU | GLN | GLY | LEU | LEU | ASP | LYS | LEU | ||||
| 26 | LYS | ASN | LYS | TRP | TRP | TYR | ASP | LYS | GLY | GLU | ||||
| 27 | CYS | GLY | SER |
Samples:
sample_1: ionotropic glutamate receptor 2, [U-98% 13C; U-98% 15N; U-95% 2H], 0.45 – 0.65 mM
condition_1: pH: 5.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HN(CO)CACB | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
Software:
nmrDraw - processing
nmrPipe - processing
Sparky - visualization
AutoAssign - automatic sequence assignment
NMR spectrometers:
- Varian Inova 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts