BMRB Entry 51831

Name: Backbone assignment of STIM1 CC3 double mutant (L416S + L423S)
Published: 2023-02-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51831

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone assignment of STIM1 CC3 double mutant (L416S + L423S)

Deposition date: 2023-02-15 Original release date: 2023-02-17

Authors: Gupta, Agrim; Baumgartner, Manuel; Muller, Norbert

Citation: Gupta, Agrim; Baumgartner, Manuel; Muller, Norbert. "1H, 13C, 15N backbone assignment of human STIM1 CC3 double mutant" .

Assembly members:
entity_1, polymer, 45 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX 4T-1

Entity Sequences (FASTA):
entity_1: GSNTLFGTFHVAHSSSLDDV DHKILTAKQASSEVTAASRE RLHRW

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	116
15N chemical shifts	39
1H chemical shifts	39

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	STIM1 CC3 double mutant	1

Entities:

Entity 1, STIM1 CC3 double mutant 45 residues - Formula weight is not available

1

GLY

SER

ASN

THR

LEU

PHE

GLY

THR

PHE

HIS

2

VAL

ALA

HIS

SER

LEU

ASP

VAL

3

ASP

HIS

LYS

ILE

LEU

THR

ALA

LYS

GLN

ALA

4

SER

GLU

VAL

THR

ALA

SER

ARG

GLU

5

ARG

LEU

HIS

ARG

TRP

Samples:

sample_1: Bis-Tris 20 mM; H20, [U-99% 2H], 10 % w/v; H20 90 % w/v; Triflouroethanol 17.5 % v/v; STIM1 CC3 double mutant, [U-95% 13C; U-90% 15N], 0.2 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CARA v1.8.4.2 - chemical shift assignment

TOPSPIN v3.5, 3.6 - collection

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts