BMRB Entry 51871
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51871
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H, 13C, and 15N backbone NMR resonance assignments of TANGO1 (30-139) from Drosophila melanogaster PubMed: 37080980
Deposition date: 2023-03-07 Original release date: 2023-03-08
Authors: Arnolds, Oliver; Stoll, Raphael
Citation: Arnolds, Oliver; Stoll, Raphael. "Characterization of a fold in TANGO1 evolved from SH3 domains for the export of bulky cargos" Nat. Commun. 14, 2273-2273 (2023).
Assembly members:
entity_1, polymer, 111 residues, 12115.1615 Da.
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE40
Entity Sequences (FASTA):
entity_1: MTLTWAATLSDKRLCADPKC
EQIISMGIAKITYAIGGEGL
ISFKINSPIRVLSKSAGSNM
QLWGVDINGRRGYANKDFIM
EKKILVRDKDLLYEVPVVGP
GSPVQSVETPV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 327 |
| 15N chemical shifts | 108 |
| 1H chemical shifts | 316 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TANGO DROME | 1 |
Entities:
Entity 1, TANGO DROME 111 residues - 12115.1615 Da.
| 1 | MET | THR | LEU | THR | TRP | ALA | ALA | THR | LEU | SER | ||||
| 2 | ASP | LYS | ARG | LEU | CYS | ALA | ASP | PRO | LYS | CYS | ||||
| 3 | GLU | GLN | ILE | ILE | SER | MET | GLY | ILE | ALA | LYS | ||||
| 4 | ILE | THR | TYR | ALA | ILE | GLY | GLY | GLU | GLY | LEU | ||||
| 5 | ILE | SER | PHE | LYS | ILE | ASN | SER | PRO | ILE | ARG | ||||
| 6 | VAL | LEU | SER | LYS | SER | ALA | GLY | SER | ASN | MET | ||||
| 7 | GLN | LEU | TRP | GLY | VAL | ASP | ILE | ASN | GLY | ARG | ||||
| 8 | ARG | GLY | TYR | ALA | ASN | LYS | ASP | PHE | ILE | MET | ||||
| 9 | GLU | LYS | LYS | ILE | LEU | VAL | ARG | ASP | LYS | ASP | ||||
| 10 | LEU | LEU | TYR | GLU | VAL | PRO | VAL | VAL | GLY | PRO | ||||
| 11 | GLY | SER | PRO | VAL | GLN | SER | VAL | GLU | THR | PRO | ||||
| 12 | VAL |
Samples:
sample_1: TANGO_DROME, [U-100% 13C; U-100% 15N], 1.0000 mM; sodium azide 0.0002 kg/m3; NaCl 137.0000 mM; KCl 2.7000 mM; Disodiumhydrogenphosphate 10.0000 mM; Potassium dihydrogen phosphate 1.8000 mM
sample_conditions_1: ionic strength: 166.500 mM; pH: 7.400; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| HNcoCACB (H[N[co[{CA|ca[C]}]]]) | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| HNcaCO (H[N[ca[CO]]]) | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| hCCcoNH (hC_cacoNH.relayed) | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr Analysis v2.4 - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker DRX 600 MHz
- Bruker AVANCE NEO 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts