BMRB Entry 51889
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51889
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Larimichthys crocea IFNd
Deposition date: 2023-03-29 Original release date: 2024-04-03
Authors: Chen, Jingjie
Citation: Chen, Jingjie. "1H and 15N Assigned Chemical Shifts for Larimichthys crocea interferon d" J. Immunol. ., .-..
Assembly members:
entity_1, polymer, 185 residues, Formula weight is not available
Natural source: Common Name: large yellow croaker Taxonomy ID: 215358 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Larimichthys crocea
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: MLSRILFVCLSLSLYSAGSS
LSCRWVDHKFRQHSETSLDL
LNTMANNSTNSTEDAQVEDT
VAFPNDLYSQASKASAEDKL
HFTVQVLEEAAALFEEDHSN
ASWEENTVENFVNVVNQQAD
GLRSCTGSHGHKKKNKKLHM
YFKRLSSHVLKKMSHSAEAW
ELIRKEIRTHLMRADQLVSS
LRNTN
- spectral_peak_list
| Data type | Count |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | interferon | 1 |
Entities:
Entity 1, interferon 185 residues - Formula weight is not available
| 1 | MET | LEU | SER | ARG | ILE | LEU | PHE | VAL | CYS | LEU | ||||
| 2 | SER | LEU | SER | LEU | TYR | SER | ALA | GLY | SER | SER | ||||
| 3 | LEU | SER | CYS | ARG | TRP | VAL | ASP | HIS | LYS | PHE | ||||
| 4 | ARG | GLN | HIS | SER | GLU | THR | SER | LEU | ASP | LEU | ||||
| 5 | LEU | ASN | THR | MET | ALA | ASN | ASN | SER | THR | ASN | ||||
| 6 | SER | THR | GLU | ASP | ALA | GLN | VAL | GLU | ASP | THR | ||||
| 7 | VAL | ALA | PHE | PRO | ASN | ASP | LEU | TYR | SER | GLN | ||||
| 8 | ALA | SER | LYS | ALA | SER | ALA | GLU | ASP | LYS | LEU | ||||
| 9 | HIS | PHE | THR | VAL | GLN | VAL | LEU | GLU | GLU | ALA | ||||
| 10 | ALA | ALA | LEU | PHE | GLU | GLU | ASP | HIS | SER | ASN | ||||
| 11 | ALA | SER | TRP | GLU | GLU | ASN | THR | VAL | GLU | ASN | ||||
| 12 | PHE | VAL | ASN | VAL | VAL | ASN | GLN | GLN | ALA | ASP | ||||
| 13 | GLY | LEU | ARG | SER | CYS | THR | GLY | SER | HIS | GLY | ||||
| 14 | HIS | LYS | LYS | LYS | ASN | LYS | LYS | LEU | HIS | MET | ||||
| 15 | TYR | PHE | LYS | ARG | LEU | SER | SER | HIS | VAL | LEU | ||||
| 16 | LYS | LYS | MET | SER | HIS | SER | ALA | GLU | ALA | TRP | ||||
| 17 | GLU | LEU | ILE | ARG | LYS | GLU | ILE | ARG | THR | HIS | ||||
| 18 | LEU | MET | ARG | ALA | ASP | GLN | LEU | VAL | SER | SER | ||||
| 19 | LEU | ARG | ASN | THR | ASN |
Samples:
sample_1: interferon, [U-95% 13C; U-95% 15N], 500 mM; NaCl 150 mM; Tris-HCl 50 mM
sample_conditions_1: pH: 7.5; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - chemical shift assignment, collection
NMR spectrometers:
- Bruker DMX 800 MHz