BMRB Entry 51901
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51901
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Title: Tst2 PubMed: 37640250
Deposition date: 2023-04-09 Original release date: 2023-05-10
Authors: Elnahriry, Khaled; Wai, Dorothy; Norton, Raymond
Citation: Elnahriry, Khaled; Wai, Dorothy; Ashwood, Lauren; Naseem, Muhammad Umair; Szanto, Tibor; Guo, Shaodong; Panyi, Gyorgy; Prentis, Peter; Norton, Raymond. "Structural and functional characterisation of Tst2, a novel TRPV1 inhibitory peptide from the Australian sea anemone Telmatactis stephensoni" Biochim. Biophys. Acta Proteins Proteom. 1872, 140952-140952 (2023).
Assembly members:
entity_1, polymer, 39 residues, 4022.7 Da.
Natural source: Common Name: Telmatactis stephensoni Taxonomy ID: 2835637 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Telmatactis stephensoni
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a
Entity Sequences (FASTA):
entity_1: GCKGQDAPCSKSKDCCGEAS
MCSKGADGKKTCMIDKLWG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 247 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tst2 | 1 |
Entities:
Entity 1, Tst2 39 residues - 4022.7 Da.
| 1 | GLY | CYS | LYS | GLY | GLN | ASP | ALA | PRO | CYS | SER | ||||
| 2 | LYS | SER | LYS | ASP | CYS | CYS | GLY | GLU | ALA | SER | ||||
| 3 | MET | CYS | SER | LYS | GLY | ALA | ASP | GLY | LYS | LYS | ||||
| 4 | THR | CYS | MET | ILE | ASP | LYS | LEU | TRP | GLY |
Samples:
sample_1: Tst2, [U-13C; U-15N], 550 uM
sample_conditions_1: ionic strength: 0.55 mM; pH: 4.7; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.6.3 - collection, processing
SPARKY v3.134 - chemical shift assignment, data analysis, peak picking
NMRPipe - processing
CYANA v3.98.13 - structure solution
X-PLOR NIH v2.48 - refinement
NMR spectrometers:
- Bruker AVANCE III 600 MHz