BMRB Entry 51906

Name: J domain of EMCV IRES J-K-St
Published: 2023-08-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51906

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: J domain of EMCV IRES J-K-St PubMed: 37640715

Deposition date: 2023-04-14 Original release date: 2023-08-09

Authors: Imai, Shunsuke; Shimada, Ichio

Citation: Imai, Shunsuke; Suzuki, Hiroshi; Fujiyoshi, Yoshinori; Shimada, Ichio. "Dynamically regulated two-site interaction of viral RNA to capture host translation initiation factor" Nat. Commun. 14, 4977-4977 (2023).

Assembly members:
entity_1, polymer, 39 residues, Formula weight is not available

Natural source: Common Name: Encephalomyocarditis virus Taxonomy ID: 12104 Superkingdom: Viruses Kingdom: not available Genus/species: Cardiovirus Cardiovirus A

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
entity_1: GGGCAGAAGGUACCCCAUUG UAUGGGAUCUGAUCUGCCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	18
1H chemical shifts	18

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Jdomain	1

Entities:

Entity 1, Jdomain 39 residues - Formula weight is not available

1

G

C

A

G

A

G

2

U

A

C

A

U

G

3

U

A

U

G

A

U

C

U

4

G

A

U

C

U

G

C

Samples:

sample_1: Jdomain, [U-2H,{13C8,1H2,1H8}-A, {13C8,1H8}-G], 1 mM; D2O 100%; potassium phosphate 10 mM; sodium chloride 10 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.4; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C TROSY aromatic	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - chemical shift assignment, peak picking, processing

NMR spectrometers:

Bruker AVANCE III 800 MHz