BMRB Entry 51947
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51947
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Citation: Zhang, Haoran; Li, Yan. "Backbone and partial side-chain assignment of the TAD domain (366-400) of human WW domain-containing transcription regulator protein 1 (TAZ/WWTR1)" .
Assembly members:
entity_1, polymer, 37 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b
Entity Sequences (FASTA):
entity_1: GSGTNVDLGTLESEDLIPLF
NDVESALNKSEPFLTWL
| Data type | Count |
| 13C chemical shifts | 105 |
| 15N chemical shifts | 36 |
| 1H chemical shifts | 138 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TAZ TAD domain (366-400) | 1 |
Entities:
Entity 1, TAZ TAD domain (366-400) 37 residues - Formula weight is not available
The recombinant TAZ TAD domain (366-400) was expressed using a pET-22b plasmid incorporated with SUMO tag for improved protein expression, with SUMO cut later for NMR characterization. TAZ TAD domain (366-400) begins with sequence GTN..., and the upstream GS is supposed to be the remaining SUMO tag.
| 1 | GLY | SER | GLY | THR | ASN | VAL | ASP | LEU | GLY | THR | ||||
| 2 | LEU | GLU | SER | GLU | ASP | LEU | ILE | PRO | LEU | PHE | ||||
| 3 | ASN | ASP | VAL | GLU | SER | ALA | LEU | ASN | LYS | SER | ||||
| 4 | GLU | PRO | PHE | LEU | THR | TRP | LEU |
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