BMRB Entry 51957
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51957
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Title: Human eIF4B C-terminal region PubMed: 37368134
Deposition date: 2023-05-07 Original release date: 2023-05-13
Authors: Mondal, Somnath; Rousseau, Sabrina; Aznauryan, Mikayel; Mackereth, Cameron
Citation: Mondal, Somnath; Rousseau, Sabrina; Talenton, Vincent; Thiam, Cheikh Ahmadou Bamba; Aznauryan, Mikayel; Mackereth, Cameron. "Backbone resonance assignments of the C-terminal region of human translation initiation factor eIF4B" Biomol. NMR Assign. 17, 199-203 (2023).
Assembly members:
entity_1, polymer, 281 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: 2B-T
Entity Sequences (FASTA):
entity_1: GCKLNLKPRSTPKEDDSSAS
TSQSTRAASIFGGAKPVDTA
AREREVEERLQKEQEKLQRQ
LDEPKLERRPRERHPSWRSE
ETQERERSRTGSESSQTGTS
TTSSRNARRRESEKSLENET
LNKEEDCHSPTSKPPKPDQP
LKVMPAPPPKENAWVKRSSN
PPARSQSSDTEQQSPTSGGG
KVAPAQPSEEGPGRKDENKV
DGMNAPKGQTGNSSRGPGDG
GNRDHWKESDRKDGKKDQDS
RSAPEPKKPEENPASKFSSA
SKYAALSVDGEDENEGEDYA
E
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 534 |
| 15N chemical shifts | 267 |
| 1H chemical shifts | 243 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | eIF4B-CTR | 1 |
Entities:
Entity 1, eIF4B-CTR 281 residues - Formula weight is not available
Gly-Cys- was added at the N-terminus
| 1 | GLY | CYS | LYS | LEU | ASN | LEU | LYS | PRO | ARG | SER | ||||
| 2 | THR | PRO | LYS | GLU | ASP | ASP | SER | SER | ALA | SER | ||||
| 3 | THR | SER | GLN | SER | THR | ARG | ALA | ALA | SER | ILE | ||||
| 4 | PHE | GLY | GLY | ALA | LYS | PRO | VAL | ASP | THR | ALA | ||||
| 5 | ALA | ARG | GLU | ARG | GLU | VAL | GLU | GLU | ARG | LEU | ||||
| 6 | GLN | LYS | GLU | GLN | GLU | LYS | LEU | GLN | ARG | GLN | ||||
| 7 | LEU | ASP | GLU | PRO | LYS | LEU | GLU | ARG | ARG | PRO | ||||
| 8 | ARG | GLU | ARG | HIS | PRO | SER | TRP | ARG | SER | GLU | ||||
| 9 | GLU | THR | GLN | GLU | ARG | GLU | ARG | SER | ARG | THR | ||||
| 10 | GLY | SER | GLU | SER | SER | GLN | THR | GLY | THR | SER | ||||
| 11 | THR | THR | SER | SER | ARG | ASN | ALA | ARG | ARG | ARG | ||||
| 12 | GLU | SER | GLU | LYS | SER | LEU | GLU | ASN | GLU | THR | ||||
| 13 | LEU | ASN | LYS | GLU | GLU | ASP | CYS | HIS | SER | PRO | ||||
| 14 | THR | SER | LYS | PRO | PRO | LYS | PRO | ASP | GLN | PRO | ||||
| 15 | LEU | LYS | VAL | MET | PRO | ALA | PRO | PRO | PRO | LYS | ||||
| 16 | GLU | ASN | ALA | TRP | VAL | LYS | ARG | SER | SER | ASN | ||||
| 17 | PRO | PRO | ALA | ARG | SER | GLN | SER | SER | ASP | THR | ||||
| 18 | GLU | GLN | GLN | SER | PRO | THR | SER | GLY | GLY | GLY | ||||
| 19 | LYS | VAL | ALA | PRO | ALA | GLN | PRO | SER | GLU | GLU | ||||
| 20 | GLY | PRO | GLY | ARG | LYS | ASP | GLU | ASN | LYS | VAL | ||||
| 21 | ASP | GLY | MET | ASN | ALA | PRO | LYS | GLY | GLN | THR | ||||
| 22 | GLY | ASN | SER | SER | ARG | GLY | PRO | GLY | ASP | GLY | ||||
| 23 | GLY | ASN | ARG | ASP | HIS | TRP | LYS | GLU | SER | ASP | ||||
| 24 | ARG | LYS | ASP | GLY | LYS | LYS | ASP | GLN | ASP | SER | ||||
| 25 | ARG | SER | ALA | PRO | GLU | PRO | LYS | LYS | PRO | GLU | ||||
| 26 | GLU | ASN | PRO | ALA | SER | LYS | PHE | SER | SER | ALA | ||||
| 27 | SER | LYS | TYR | ALA | ALA | LEU | SER | VAL | ASP | GLY | ||||
| 28 | GLU | ASP | GLU | ASN | GLU | GLY | GLU | ASP | TYR | ALA | ||||
| 29 | GLU |
Samples:
sample_1: eIF4B-CTR, [U-100% 13C; U-100% 15N], 150 uM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 2 mM
sample_conditions_1: ionic strength: 170 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HA(CACO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D N(COCA)NNH | sample_1 | isotropic | sample_conditions_1 |
| 3D HACAN | sample_1 | isotropic | sample_conditions_1 |
| 3D HACA(CO)N | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
NMRFAM-SPARKY - chemical shift assignment, data analysis
NMR spectrometers:
- Bruker AVANCE NEO 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts