BMRB Entry 51987

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51987

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Title:
1H and 15N backbone resonance assignments of the D10N,P146A variant of beta-phosphoglucomutase (trans K145-A146 peptide bond) in a Mg-bound complex with fructose 1,6-bisphosphate
Deposition date:
2023-06-05
Original release date:
2024-07-30
Authors:
Baxter, Nicola; Waltho, Jonathan
Citation:

Citation: Cruz-Navarrete, F.; Baxter, Nicola; Flinders, Adam; Buzoianu, Anamaria; Cliff, Matthew; Baker, Patrick; Waltho, Jonathan. "Peri active site catalysis of proline isomerisation is the molecular basis of allomorphy in beta-phosphoglucomutase"  Commun. Biol. 7, 909-909 (2024).
PubMed: 39068257

Assembly members:

Assembly members:
entity_1, polymer, 221 residues, Formula weight is not available
entity_FBP, non-polymer, 340.116 Da.
entity_MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Lactococcus lactis   Taxonomy ID: 1358   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Lactococcus lactis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MFKAVLFDLNGVITDTAEYH FRAWKALAEEIGINGVDRQF NEQLKGVSREDSLQKILDLA DKKVSAEEFKELAKRKNDNY VKMIQDVSPADVYPGILQLL KDLRSNKIKIALASASKNGP FLLERMNLTGYFDAIADPAE VAASKAAPDIFIAAAHAVGV APSESIGLEDSQAGIQAIKD SGALPIGVGRPEDLGDDIVI VPDTSHYTLEFLKEVWLQKQ K

Data sets:
Data typeCount
15N chemical shifts196
1H chemical shifts196

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1bPGM-D10N,P146A1
2F16BP2
3Mg3

Entities:

Entity 1, bPGM-D10N,P146A 221 residues - Formula weight is not available

M1-F2-K3-A4-....-K221

1   METPHELYSALAVALLEUPHEASPLEUASN
2   GLYVALILETHRASPTHRALAGLUTYRHIS
3   PHEARGALATRPLYSALALEUALAGLUGLU
4   ILEGLYILEASNGLYVALASPARGGLNPHE
5   ASNGLUGLNLEULYSGLYVALSERARGGLU
6   ASPSERLEUGLNLYSILELEUASPLEUALA
7   ASPLYSLYSVALSERALAGLUGLUPHELYS
8   GLULEUALALYSARGLYSASNASPASNTYR
9   VALLYSMETILEGLNASPVALSERPROALA
10   ASPVALTYRPROGLYILELEUGLNLEULEU
11   LYSASPLEUARGSERASNLYSILELYSILE
12   ALALEUALASERALASERLYSASNGLYPRO
13   PHELEULEUGLUARGMETASNLEUTHRGLY
14   TYRPHEASPALAILEALAASPPROALAGLU
15   VALALAALASERLYSALAALAPROASPILE
16   PHEILEALAALAALAHISALAVALGLYVAL
17   ALAPROSERGLUSERILEGLYLEUGLUASP
18   SERGLNALAGLYILEGLNALAILELYSASP
19   SERGLYALALEUPROILEGLYVALGLYARG
20   PROGLUASPLEUGLYASPASPILEVALILE
21   VALPROASPTHRSERHISTYRTHRLEUGLU
22   PHELEULYSGLUVALTRPLEUGLNLYSGLN
23   LYS

Entity 2, F16BP - C6 H14 O12 P2 - 340.116 Da.

1   FBP

Entity 3, Mg - Mg - 24.305 Da.

1   MG

Download HSQC peak lists in one of the following formats:
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