BMRB Entry 52067

Name: Chemical shift assignments for N-Myc 64-137
Published: 2024-10-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52067

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignments for N-Myc 64-137

Deposition date:

2023-08-02

Original release date:

2024-10-08

Authors:

Rejnowicz, Ewa; Batchelor, Matthew; Leen, Eoin; Bayliss, Richard

Citation:

Citation: Rejnowicz, Ewa; Batchelor, Matthew; Leen, Eoin; Ahangar, Mohd Syed; Burgess, Selena; Richards, Mark; Kalverda, Arnout; Bayliss, Richard. "Exploring the dynamics and interactions of the N-myc transactivation domain through solution NMR" Biochem. J. ., .-. (2024).
PubMed: 39370942

Assembly members:

Assembly members:
entity_1, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM6T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGSRGFAEHSSEPPSWVT EMLLENELWGSPAEEDAFGL GGLGGLTPNPVILQDCMWSG FSAREKLERAVSEKLQHG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	218
15N chemical shifts	71
1H chemical shifts	71

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	N-Myc 64-137	1

Entities:

Entity 1, N-Myc 64-137 78 residues - Formula weight is not available

Residue 5 in the sequence relates to Ser64 in N-Myc

1

GLY

ALA

MET

GLY

SER

ARG

GLY

PHE

ALA

GLU

2

HIS

SER

GLU

PRO

SER

TRP

VAL

THR

3

GLU

MET

LEU

GLU

ASN

GLU

LEU

TRP

GLY

4

SER

PRO

ALA

GLU

ASP

ALA

PHE

GLY

LEU

5

GLY

LEU

GLY

LEU

THR

PRO

ASN

PRO

6

VAL

ILE

LEU

GLN

ASP

CYS

MET

TRP

SER

GLY

7

PHE

SER

ALA

ARG

GLU

LYS

LEU

GLU

ARG

ALA

8

VAL

SER

GLU

LYS

LEU

GLN

HIS

GLY

Samples:

sample_1: N-Myc 64-137, [U-99% 13C; U-99% 15N], 200 uM; TRIS 25 mM; sodium chloride 137 mM; potassium chloride 2.7 mM; D2O 5%; TCEP 2 mM

sample_conditions_1: ionic strength: 0.2 M; pH: 6.9; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CcpNMR vAnalysis v 2.5 - chemical shift assignment, data analysis

NMR spectrometers:

Oxford/Bruker AVANCE III 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks