BMRB Entry 52068
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52068
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Title: Chemical shift assignment of the ClpL-N-terminal domain from Listeria monocytogenes PubMed: 38598269
Deposition date: 2023-08-04 Original release date: 2024-04-05
Authors: Hennig, Janosch; Hollmann, Nele
Citation: Bohl, Valentin; Hollmann, Nele; Melzer, Tobias; Katikaridis, Panagiotis; Meins, Lena; Simon, Bernd; Fleming, Dirk; Sinning, Irmgard; Hennig, Janosch; Mogk, Axel. "The Listeria monocytogenes persistence factor ClpL is a potent stand-alone disaggregase" eLife 12, 92746-92746 (2024).
Assembly members:
entity_1, polymer, 69 residues, Formula weight is not available
Natural source: Common Name: Listeria monocytogenes Taxonomy ID: 1639 Superkingdom: Bacteria Kingdom: not available Genus/species: Listeria monocytogenes
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a
Entity Sequences (FASTA):
entity_1: MANFYGNDPFFNNDMDDVFN
QLFRRMDNQNSERARYLVNG
QSLTPDEFTQYRATGKLPQN
NLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 214 |
| 15N chemical shifts | 55 |
| 1H chemical shifts | 355 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ClpL-NTD | 1 |
Entities:
Entity 1, ClpL-NTD 69 residues - Formula weight is not available
| 1 | MET | ALA | ASN | PHE | TYR | GLY | ASN | ASP | PRO | PHE | ||||
| 2 | PHE | ASN | ASN | ASP | MET | ASP | ASP | VAL | PHE | ASN | ||||
| 3 | GLN | LEU | PHE | ARG | ARG | MET | ASP | ASN | GLN | ASN | ||||
| 4 | SER | GLU | ARG | ALA | ARG | TYR | LEU | VAL | ASN | GLY | ||||
| 5 | GLN | SER | LEU | THR | PRO | ASP | GLU | PHE | THR | GLN | ||||
| 6 | TYR | ARG | ALA | THR | GLY | LYS | LEU | PRO | GLN | ASN | ||||
| 7 | ASN | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: ClpL-NTD, [U-99% 13C; U-99% 15N], 410 uM; sodium phosphate 50 mM; potassium chloride 50 mM; dithiothreitol 2 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts