BMRB Entry 52092

Name: Solution NMR chemical shift assignments for a subdomain construct of 7SK SL3 RNA in the SL3a state
Published: 2023-09-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52092

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution NMR chemical shift assignments for a subdomain construct of 7SK SL3 RNA in the SL3a state PubMed: 37609139

Deposition date: 2023-08-22 Original release date: 2023-09-11

Authors: Eichhorn, Catherine

Citation: Camara, Momodou; Lange, Bret; Yesselman, Joseph; Eichhorn, Catherine. "Visualizing a two-state conformational ensemble in stem-loop 3 of the transcriptional regulator 7SK RNA" Nucleic Acids Res. ., .-. (2023).

Assembly members:
entity_1, polymer, 38 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):
entity_1: GGCUCCAAACAAGCUCUCAA GGUCCAUUUGUAGGAGCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	78
15N chemical shifts	12
1H chemical shifts	118

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SL3a-top hairpin	1

Entities:

Entity 1, SL3a-top hairpin 38 residues - Formula weight is not available

1

G

C

U

C

A

C

2

A

G

C

U

C

U

C

A

3

G

U

C

A

U

G

4

U

A

G

A

G

C

Samples:

sample_1: SL3a-top hairpin 0.8 mM; SL3a-top hairpin, [U-100% 13C; U-100% 15N], 0.8 mM; Sodium Phosphate 20 mM; Potassium Chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz