BMRB Entry 52138

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52138

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Title:
Backbone assignment of the R178Q mutant of ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1)
Deposition date:
2023-09-19
Original release date:
2024-01-09
Authors:
Chiang, Tsung-Sheng; Lai, Chih-Hsuan; Hsu, Shang-Te Danny
Citation:

Citation: Kenny, Sebastian; Lai, Chih-Hsuan; Chiang, Tsung-Sheng; Brown, Kwame; Hewitt, Chad; Krabill, Aaron; Chang, Hao-Ting; Wang, Yong-Sheng; Flaherty, Daniel; Danny Hsu, Shang-Te; Das, Chittaranjan. "Altered protein dynamics and a more reactive catalytic cysteine in a neurodegeneration-associated UCHL1 mutant"  J. Mol. Biol. 436, 168438-168438 (2024).
PubMed: 38185323

Assembly members:

Assembly members:
entity_1, polymer, 231 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MQLKPMEINPEMLNKVLSRL GVAGQWRFVDVLGLEEESLG SVPAPACALLLLFPLTAQHE NFRKKQIEELKGQEVSPKVY FMKQTIGNSCGTIGLIHAVA NNQDKLGFEDGSVLKQFLSE TEKMSPEDRAKCFEKNEAIQ AAHDAVAQEGQCRVDDKVNF HFILFNNVDGHLYELDGQMP FPVNHGASSEDTLLKDAAKV CREFTEREQGEVRFSAVALC KAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts506
15N chemical shifts172
1H chemical shifts172

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1UCHL1_R178Q1

Entities:

Entity 1, UCHL1_R178Q 231 residues - Formula weight is not available

1   METGLNLEULYSPROMETGLUILEASNPRO
2   GLUMETLEUASNLYSVALLEUSERARGLEU
3   GLYVALALAGLYGLNTRPARGPHEVALASP
4   VALLEUGLYLEUGLUGLUGLUSERLEUGLY
5   SERVALPROALAPROALACYSALALEULEU
6   LEULEUPHEPROLEUTHRALAGLNHISGLU
7   ASNPHEARGLYSLYSGLNILEGLUGLULEU
8   LYSGLYGLNGLUVALSERPROLYSVALTYR
9   PHEMETLYSGLNTHRILEGLYASNSERCYS
10   GLYTHRILEGLYLEUILEHISALAVALALA
11   ASNASNGLNASPLYSLEUGLYPHEGLUASP
12   GLYSERVALLEULYSGLNPHELEUSERGLU
13   THRGLULYSMETSERPROGLUASPARGALA
14   LYSCYSPHEGLULYSASNGLUALAILEGLN
15   ALAALAHISASPALAVALALAGLNGLUGLY
16   GLNCYSARGVALASPASPLYSVALASNPHE
17   HISPHEILELEUPHEASNASNVALASPGLY
18   HISLEUTYRGLULEUASPGLYGLNMETPRO
19   PHEPROVALASNHISGLYALASERSERGLU
20   ASPTHRLEULEULYSASPALAALALYSVAL
21   CYSARGGLUPHETHRGLUARGGLUGLNGLY
22   GLUVALARGPHESERALAVALALALEUCYS
23   LYSALAALALEUGLUHISHISHISHISHIS
24   HIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks